大鼠棕色和白色脂肪干细胞体外成脂能力的比较  

Comparison of in vitro lipogenic potential of rat brown and white adipose stem cells

作　　者：冯顶丽 卓丽丹 芦笛[5] 高杨 李红[3] 郭红延[2] FENG Ding-li;ZHUO Li- dan;LU Di;GAO Yang;LI Hong;GUO Hong-yan(Shanxi Medical University School and Hospital of Stomatology,Shanxi Taiyuan 030001,China;Stomatology Center,Beijing Armed Police General Hospital,Beijing 100039,China;Jinzhou Medical University,Liaoning Jinzhou 121000,China;Institute of Military Medicine,Academy of Military Sciences,Belting 100850,China;General Hospital of Chinese People＇s Liberation Army,Beijing 100853,China;Fushun Central Hospital,Liaoning Fushun 113006,China.)

机构地区：[1]山西医科大学口腔医学院.口腔医院,山西太原030001 [2]北京武警总医院口腔医学中心,北京100039 [3]军事科学院军事医学研究院,北京100850 [4]锦州医科大学,辽宁锦州121000 [5]中国人民解放军总医院,北京100853 [6]抚顺市中心医院,辽宁抚顺113006

出　　处：《临床口腔医学杂志》2018年第7期397-401,共5页Journal of Clinical Stomatology

基　　金：国家自然科学基金[国际(地区)合作与交流项目(313101087)];国家自然科学基金(81571619);国家重点研发计划(2016 YFE0204400)

摘　　要：目的:比较棕色脂肪干细胞(brown adipose stem cells,BADSCs)和白色脂肪干细胞(white adipose stem cells,WADSCs)的体外成脂能力。方法:分离SD大鼠脂肪组织,获得BADSCs和WADSCs。显微镜观察细胞形态、流式鉴定细胞表面抗原标记物,并成脂诱导两种细胞。诱导后油红O染色显示形成的脂滴。RT-PCR检测成脂相关基因的表达。结果:BADSCs和WADSCs都具有成脂分化能力;与BADSCs相比,WADSCs脂滴形成相对较快且数量较多,RT-PCR结果显示PPAR、C/EBPβ成脂标志基因表达也相对较高。结论:BADSCs和WADSCs都具有成脂分化能力。相比较而言,WADSCs有较强的成脂分化能力。Objective： To compare the adipogenic capacity of brown adipose derived stem cells（ BADSCs） and white adipose stem cells（ WADSCs）. Methods： SD rat adipose tissue was isolated and BADSCs and WADSCs were obtained. Microscopic observation of cell morphology,flow cytometric identification of cell surface antigen markers,and adipogenic induction of both cells. Oil Red-O staining after induction showed the formation of lipid droplets. RT-PCR detected the expression of adipogenic related genes. Results： Both BADSCs and WADSCs had adipogenic differentiation ability. Compared with BADSCs,the formation of droplets in WADSCs was relatively rapid and the number was large. RT-PCR results showed that the PPAR and C/EBPβ adipogenic marker genes were also relatively high. Conclusion： Both BADSCs and WADSCs have adipogenic differentiation ability. In comparison,WADSCs have strong adipogenic differentiation.

关 键 词：棕色脂肪干细胞 白色脂肪干细胞 成脂分化 组织工程 

分 类 号：R813.1[医药卫生—放射医学] R318[医药卫生—临床医学]