曝气水中邻氨基苯酚的HPLC检测方法  

Determination of O-Aminophenol in Aerated Water by HPLC Method

作　　者：陈鸿申 肖飞 王睿 张明生[1] CHEN Hong-shen;WANG Rui;XIAO Fei;ZHANG Ming-sheng(College of Life Sciences,Guizhou University,Guiyang,Guizhou 550025,China;Guizhou Academy of Testing and Analysis Guiyang,Guizhou 550002,China)

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州省分析测试研究院,贵州贵阳550002

出　　处：《山地农业生物学报》2018年第2期85-88,共4页Journal of Mountain Agriculture and Biology

基　　金：贵州省科技创新人才团队建设专项资金(黔科合平台人才[2016]5624);贵州省教育厅创新群体重大研究项目(黔教合KY字[2016]023和生物工程企业合作项目(备案号:HD2014200070001)

摘　　要：建立了高效液相色谱检测曝气水中邻氨基苯酚的方法。采用Thermo Hypersil GOLD色谱柱(250×4.6,5μm,part No.25005-254630),选用48%∶52%水-乙腈为流动相,在波长234 nm、流速1.0 m L/min、柱温为25℃操作条件下以外标法进行定量分析。试验结果表明,邻氨基苯酚质量浓度在0.2~10.0μg/m L范围内线性关系良好(y=30535 x-1708.8,R^2=0.9997)。此外,本方法建立的加标回收率(98.0%~104.8%)、重复性(RSDArea=0.36%)、准确度(0.06%~1.7%)、检出限(0.08 mg/L)和定量限(0.27 mg/L)等试验结果均满足定性分析和定量分析。该方法具有易操作、分离特性好的特点。A method for the determinationin in aerated water of o-Aminophenol was established by HPLC.HPLC was carried out on a GOLD Thermo Hypersil column （250mm×4.6mm,5 μm,part No. 25005-254630）,using water and acetonitrile （48%：52%） as mobile phase and the detection was performed by UV detector at 234 nm,of which the flow rate was 1.0 mL/min and the column temperature was 25 ℃ through the external standard method. The results showed that the o-aminophenol concentration ranged from 0.2μg/mL to 10.0 μg/mL can obtained a good linear relationship （y= 30535x-1708.8？ R2= 0.9997） . In addi-tion,the results of adding standard recovery （98.0%~104.8%）,repeatability （RSDArea=0.36%）,accu-racy （0.06%~1.7%）,LOD （0.08mg/L） and LOQ （0.27mg/L） could good meet the qualitative analysis and quantitative analysis. Detection methods for o-aminophenol are easy to operate,the characteristics of sep-aration properties are good.

关 键 词：曝气水 邻氨基苯酚 高效液相色谱法 检测方法 

分 类 号：O625.3[理学—有机化学]