miRNA-145调控靶基因c-Myc抑制胃癌增殖、侵袭  

miR-145 Inhibite Tumor Proliferation and Invasion in Gastric Cancer by Targeting c-Myc

作　　者：彭伟辉 黄江生[1] 段伦喜[1] 张磊屹[1] 左仲坤 唐腾龙 Peng Weihui;Huang Jiangsheng;Duan Lunxi(The Department of Minimally Invasive Surgery,The Second Xiangya Hospital of Central South University,Hunan 410000,China)

机构地区：[1]中南大学湘雅二医院微创外科,长沙410001

出　　处：《医学研究杂志》2018年第5期60-64,共5页Journal of Medical Research

基　　金：湖南省科学技术厅基金资助项目(2016jc2048)

摘　　要：目的探索microRNA145(miR-145)对胃癌细胞增殖活性的影响,及与其靶基因c-Myc的调控关系。方法选取人胃癌细胞株SGC-7901作为研究对象,并向细胞株内转染miR-145模拟物和miR-145阴性对照物,荧光显微镜观察、QPCR检测转染后细胞株miR-145的变化,并通过细胞软琼脂克隆增殖实验和MTT实验检测转染后SGC-7901细胞株的增殖能力、Transwell侵袭小室检测细胞侵袭能力。Western blot法检测转染后SGC-7901细胞株中c-Myc蛋白的表达情况。结果转染后,miR-145模拟物组中miR-145的表达明显高于miR-145阴性对照和空白组,转染miR-145模拟物后SGC-7901细胞增殖能力显著降低(P<0.05),c-Myc蛋白表达也明显较低(P<0.05)。结论 miR-145能通过抑制靶基因c-Myc的表达,抑制胃癌细胞的增殖与侵袭能力。总之,miR-145可能在胃癌中发挥抑癌基因的作用,是胃癌潜在的早期诊断指标和治疗靶点。Objective To investigate the effect of miR-145 on proliferation of gastric cancer,And its relationship with target gene c-Myc.Methods Human gastric cancer cell line SGC-7901 was selected as study object.miR-145 mimic and miR-145 NC were synthesised and transfected into SGC-7901 cells.Q-PCR was used to detect the relative expression levels of miR-145.The effects of miR-145 on proliferation and invasion were detected by colony formation and MTT assay and Transwell invasion chambers.The expression of c-Myc were detected by Western blot.Results miR-145 were successfully transfected into SGC-7901 cells.The number of colony-forming units was obviously lower than the miR-145 NC group and Normal group（P 〈0.05）.The expression of c-Myc protein in miR145 mimic group were significantly lower than miR-145 NC group and Normal group（P 〈0.05）.Conclusion Therefore,we concluded that miRNA-145 can inhibit cell proliferation and invasion,and the partial mechanism may relate to the inhibition of the expression of c-Myc protein.In brief,miRNA-145 may play a role of cancer suppressor gene and it may become a new early diagnosis mark and therapy target in gastric cancer.

关 键 词：胃癌 MIR-145 C-MYC 增殖 

分 类 号：R4[医药卫生—临床医学]