多巴胺改性海藻酸多孔支架的制备与性能  

Preparation and Properties of Polydopamine and Alginate Porous Complex Scaffolds

作　　者：章朱迎 陈静 俞佳蕾 赵倩 曹传昊 沈佳丽 施冬健 ZHANG Zhuying;CHEN Jing;YU Jialei;ZHAO Qian;CAO Chuanhao;SHEN Jiali;SHI Dongjian(Key Laboratory of Synthetic and Biological Colloids,Ministry of Education,School of Chemical and Material Engineering,Jiangnan University,Wuxi,Jiangsu 214122,China)

机构地区：[1]江南大学化学与材料工程学院,合成与胶体生物教育部重点实验室,江苏无锡214122

出　　处：《应用化学》2018年第6期665-673,共9页Chinese Journal of Applied Chemistry

基　　金：江苏省高等学校大学生创新创业训练计划项目(201610295019Y)

摘　　要：生物大分子海藻酸(Alg)由于其安全、无毒、可生物降解等特性而被广泛应用于组织工程领域。受海洋贻贝蛋白结构的启发,多巴胺(DA)具有优异的粘附性能,在碱性水溶液条件下可发生氧化自聚形成聚多巴胺(PDA)。以Alg为基体,加入PDA纳米粒子复合,并通过冷冻干燥法制备得到Alg/PDA多孔支架材料。结果表明,Alg/PDA多孔支架材料具有较为规整的内部结构。改变Alg质量浓度,Alg/PDA支架材料的孔径可控制在60~120μm之间,孔隙率可控于80%~88%。所得的支架材料具有适宜大小的孔径和孔隙率,结果表明支架材料对细胞无毒副作用。Alginate（ Alg） is widely used in the field of tissue engineering because of its safe,non-toxic and biodegradable properties. Inspired by mussel adhesive protein, dopamine（ DA） has excellent adhesion property and can self-oxidize to polydopamine（ PDA） in weak basic buffer solution. Alg/PDA porous scaffolds were prepared by freeze-dry of Alg and PDA mixture. The results show that Alg/PDA porous scaffolds have relatively homogeneous internal structure. By changing the mass concentration of Alg,the pore size of the Alg/PDA complex scaffolds can be limited to 60 ~ 120 μm,and the porosity of the scaffolds can be controlled in the range of 80% ~ 88% which is appropriate for the growth of bond cells. The biocompatibility results indicate that the scaffolds show low cytotoxicity.

关 键 词：多巴胺 海藻酸 多孔支架材料 

分 类 号：O636[理学—高分子化学]