2,6-dansyl azide快速测定血清中H_2S含量  

Rapid determination of serum hydrogen sulfide level by 2,6-dansyl azide

作　　者：付林 邹小艳[1] 马建滨 都玉蓉 郭松长[1,5] FU Lin;ZOU Xiao-yan;MA Jian-bin;DU Yu-rong;GUO Song-chang(Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810007;University of Chinese Academy of Science,Beijing 100049;School of Life Science,Qinghai Normal University,Xining 810008;Key Laboratory of Medicinal Plant and Animal Resources the Qinghai-Tibetan Plateau in Qinghai Province,Xining 810008;Hunan Collaborative Inovation Center of Animal Production Safey,College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128)

机构地区：[1]中国科学院西北高原生物研究所,西宁810007 [2]中国科学院大学,北京100049 [3]青海师范大学生命科学学院,西宁810008 [4]青海省青藏高原药用动植物资源重点实验室,西宁810008 [5]湖南农业大学动物科学技术学院湖南畜禽安全生产协同创新中心,长沙410128

出　　处：《分析试验室》2018年第8期898-901,共4页Chinese Journal of Analysis Laboratory

基　　金：青海省应用基础研究计划(2013-Z-750);国家自然科学基金(31760117);青海省青藏高原药用动植物资源重点实验室项目(2017-ZJ-Y13)资助

摘　　要：对测定血清中H2S浓度的方法进行了改进。基于2,6-dansyl azide本身不发荧光,但与H2S作用后显示强烈荧光这一特性,一步法合成2,6-dansyl azide后采用PBS/DM SO体系(pH 7.5)替代PBS/Tween-20体系(pH 7.5)测定内源性H2S。数据分析显示改进的方法具有更低的荧光背景值。应用本方法成功对6只成年健康SD大鼠的血浆H2S水平进行了测定。An improved method for measuring H2 S concentration in serum was described. The 2,6-dansyl azide（2,6-DNS-Az） itself does not fluoresce,however,it can emit a strong fluorescence when reacts with H2 S. Based on this characteristic,2,6-DNS-Az was synthesized by one-step method,then endogenous H2 S was determined with PBS buffer/DMSO（pH7. 5） instead of PBS buffer or PBS buffer/Tween-20（pH7. 5）. The study indicated that the improved assay method has a better determination effect with lower fluorescence background value. Furthermore,this method was used successfully for measuring endogenous hydrogen sulfide concentration in plasma from 6 healthy adult SD rats.

关 键 词：一步法 2 6-dansylazide H2S Buffer/DMSO缓冲体系 

分 类 号：R446[医药卫生—诊断学] O657.3[医药卫生—临床医学]