深加工阿胶制品DNA提取方法对比探究  

Comparison Study on DNA Extraction Methods of Deep Processing Products of Asini Corii Colla

作　　者：王一村 李娜 高静静 周莉莉 许士明 WANG Yi-cun, LI Na, GAO Jing-jing, ZHOU Li-li, XU Shi-ming(Shandong Institute for Product Quality Inspection/National Supervision and Inspection Center for Processed Food Quality （Shandong）, Jinan 250102, China)

机构地区：[1]山东省产品质量检验研究院/国家加工食品质量监督检验中心(山东),山东济南250102

出　　处：《食品与药品》2018年第3期179-183,共5页Food and Drug

基　　金：国家质量监督检验检疫总局科技计划项目(2016QK096);2017年度中央引导地方科技发展专项资金项目

摘　　要：目的针对深加工阿胶制品,选择一种最优DNA提取方法,为动物源性真伪鉴定奠定基础。方法对5种不同的DNA提取方法进行综合比较,获取一种适合阿胶的高质量DNA提取方法。结果超高速离心法提取的DNA纯度最高,质量最好,完全能满足下游实验要求。结论超高速离心法适合深加工阿胶制品DNA提取分离。Objective To screen an optimal method for extraction of DNA from deeply processed products of Asini corii colla. Methods A high quality DNA extraction method suitable for Asini corii colla was obtained by comparative study on 5 different DNA extraction methods. Results For deep processed products of Asini corii colla, the DNA extracted by ultra-high speed centrifugation had the highest purity and the best quality, which fully met the requirements of downstream experiments. Conclusion Ultra-high speed centrifugation is suitable for DNA extraction from deeply processed products of Asini corii colla.

关 键 词：阿胶 深加工制品 DNA提取 超高速离心法 

分 类 号：R282.5[医药卫生—中药学]