HPLC测定多烯紫杉醇纳米粒包封率  

Determination of Encapsulation Efficiency of Docetaxel Nanoparticles by HPLC

作　　者：毛丽燕 邬文珠 路文娟[1,2,3] 赵刚 MAO Li-yan1,2,3, WU Wen-zhu1,2, LU Wen-juan1,2,3, ZHAO Gang1,2,3,4(1. School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250200, China; 2. Institute of Materia Medica, Shandong Academy of Medical Sciences, Jinan 250062, China; 3. Key Laboratory for Biotech-Drugs Ministry of Health, Jinan 250062, China; 4. Key Laboratory for Rare ＆ Uncommon Diseases of Shandong Province, Jinan 250062, Chin)

机构地区：[1]济南大学山东省医学科学院医学与生命科学学院,山东济南250200 [2]山东省医学科学院药物研究所,山东济南250062 [3]国家卫生部生物技术药物重点实验室,山东济南250062 [4]山东省罕少见病重点实验室,山东济南250062

出　　处：《食品与药品》2018年第3期194-197,共4页Food and Drug

基　　金：国家自然科学基金项目(No:21606147);山东省医学科学院医药卫生科技创新工程;山东省医学科学院科技计划面上项目(编号2016-13)

摘　　要：目的制备多烯紫杉醇纳米粒溶液并用高效液相色谱法(HPLC)测定包封率。方法采用乳化溶剂挥发法制备多烯紫杉醇纳米粒溶液,采用HPLC测定包封率。结果多烯紫杉醇在0.2000~3.2000 mg/ml(r=0.9999)浓度范围内线性关系良好,精密度符合要求。平均回收率为98.52%,RSD值为3.67%(n=5)。平均包封率85.15%,RSD值为3.79%。结论 HPLC可用于测定该多烯紫杉醇纳米粒的包封率。Objective To prepare the docetaxel nanoparticle solution and determine its encapsulation efficiency by HPLC. Methods Emulsion solvent evaporation method was used to prepare the docetaxel nanoparticles. HPLC was adopted to determine the encapsulation efficiency. Results The linear relationship of docetaxel was good within 0.2000-3.2000 mg/ml（r=0.9999） concentration range. Precision can meet the requirements. The average recovery was 98.52 % with RSD of 3.67 %（n=5）. The average encapsulation efficiency was 85.15 % with RSD of 3.79 %. Conclusion HPLC can be used to determine the encapsulation efficiency of docetaxel nanoparticles.

关 键 词：多烯紫杉醇 纳米粒 高效液相色谱 包封率 

分 类 号：R944[医药卫生—药剂学] R917[医药卫生—药学]