HPLC测定心可宁胶囊中人工牛黄(牛黄)胆红素的含量  

Determination of Bilirubin in Artificial Bezoar or Bezoar in Xinkening Capsules by HPLC

作　　者：袁继承 张裕民 沙启营 YUAN Ji-cheng1, ZHANG Yu-min2, SHA Qi-ying2(1. Heze Medical College, Heze 274000, China; 2. Heze Inspection and Testing Institute of Drug and Food, Heze 274000, Chin)

机构地区：[1]菏泽医学专科学校,山东菏泽274000 [2]菏泽市食品药品检验检测研究院,山东菏泽274000

出　　处：《食品与药品》2018年第3期207-210,共4页Food and Drug

摘　　要：目的采用高效液相色谱法测定心可宁胶囊中人工牛黄(牛黄)胆红素的含量。方法色谱柱以十八烷基硅烷键合硅胶为填充剂(Phenomenex,4.6 mm×250 mm,5μm),以二甲基亚砜-四氢呋喃-2%冰醋酸溶液(50:47:3)为流动相,流速1.0 ml/min,检测波长445 nm,柱温30℃。结果胆红素浓度线性范围为5.05~50.53μg/m L(r=0.9999,n=6),平均加样回收率为99.86%,RSD为1.36%。测定4个厂家生产的心可宁胶囊样品,结果3个厂家胆红素含量偏低或未检出。结论此法准确、简便,可作为心可宁胶囊中人工牛黄(牛黄)胆红素的含量测定方法。Objective To establish an HPLC method for the determination of bilirubin in artificial bezoar or bezoar in Xinkening Capsules. Method The chromatographic column was loaded with octadecyl silane bonded silica（Phenomenex, 4.6 mm×250 mm, 5μm）,with DMSO-tetrahydrofuran-2 % glacial acetic acid（50：47：3） as mobile phase at the flow rate of 1.0 ml/min. The detection wavelength was 445 nm, and the column temperature was 30 ℃. Results The linear range of bilirubin was 5.05-50.53 μg/ml（r=0.9999,n=6）. The average recovery was 99.86 %, with RSD of 1.36 %. The bilirubin contents of Xinkening Capsules from four different manufacturers were detected, among which the bilirubin contents of three manufacturers＇ products were low or undetected. Conclusion This method is accurate and simple, and can be adopted in the determination of bilirubin in artificial bezoar or bezoar in Xinkening Capsules.

关 键 词：心可宁胶囊 牛黄 人工牛黄 胆红素 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]