Effect of Low Temperature Treatment on Agrobacterium-mediated Transformation of Maize Seed Buds  

作　　者：Mengyu LYU Fushuang DONG Junmin ZHANG He ZHAO Jianfang CHAI Shuo ZHOU Fan YANG Yongwei LIU Haibo WANG 

机构地区：[1]Plant Genetic Engineering Center of Hebei Province,Institute of Genetics and Physiology,Hebei Academy of Agriculture and Forestry Sciences

出　　处：《Agricultural Biotechnology》2018年第3期75-76,共2页农业生物技术（英文版）

基　　金：Supported by Natural Science Foundation of Hebei Province(C2017301071);Financial Project of Hebei Province(2017039339)

摘　　要：[Objectives]This study was conducted to improve the genetic transformation rate of maize. [Methods]The seeds of maize ＂ Zheng 58＂ as the experimental material were germinated and treated under freezing temperature of-18 ℃ for 30 and 35 min. The EGFP gene was transformed into the growth points of these seed buds by Agrobacterium tumefaciens EHA105. The transformation effect was determined by fluorescence protein detection on transformed buds. [Results]After a certain period of treatment at-18 ℃ following germination and transformation of maize bud growth points with A. tumefaciens,although the survival rate of maize buds was reduced,the percentage of transformed cells significantly increased. [Conclusions]Low temperature treatment can improve the transformation efficiency of A. tumefaciens to the growth point of maize bud.[Objectives]This study was conducted to improve the genetic transformation rate of maize. [Methods]The seeds of maize ＂ Zheng 58＂ as the experimental material were germinated and treated under freezing temperature of-18 ℃ for 30 and 35 min. The EGFP gene was transformed into the growth points of these seed buds by Agrobacterium tumefaciens EHA105. The transformation effect was determined by fluorescence protein detection on transformed buds. [Results]After a certain period of treatment at-18 ℃ following germination and transformation of maize bud growth points with A. tumefaciens,although the survival rate of maize buds was reduced,the percentage of transformed cells significantly increased. [Conclusions]Low temperature treatment can improve the transformation efficiency of A. tumefaciens to the growth point of maize bud.

关 键 词：Maize buds Low temperature treatment AGROBACTERIUM TRANSGENIC 

分 类 号：S513[农业科学—作物学] TS221[轻工技术与工程—粮食、油脂及植物蛋白工程]