Study on Extraction Method of Selenium-enriched Rice  

作　　者：Panxia LIANG Xiu LAN Yongxian LIU Liping PAN Mengling NONG Ying XING Qing LIAO Shiyang LU Jinping CHEN 

机构地区：[1]Resources and Environment Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China [2]Guangxi University, Nanning 530007,China

出　　处：《Agricultural Biotechnology》2018年第3期165-169,共5页农业生物技术（英文版）

基　　金：Supported by the Science and Technology Major Project of Guangxi(Guike AA17202026);Program for Scientific Research and Technology Development in Xixiangtant District of Nanning City(201710304);the Special Fund for the Innovation-Driven Development in Guangxi(Guike AA17202019-4&AA17202019);the Science and Technology Development Fund of Guangxi Academy of Agricultural Sciences(Guinongke 2017JM03);the Program for the Scientific Research and Technology Development in Guangxi(Guikehe415104001-22);the Fundamental Research Funds for Guangxi Academy of Agricultural Sciences(Guinongke 2017YZ03);the Key Research and Development Program of Guangxi(Guike AB16380088);the Experiment Station for Selenium Featured Crops in Guangxi(Gui TS2016011);the Key Research and Development Program of Qingxiu District,Guangxi(2016039);the Scientific and Technological Transformative Project of Guangxi Academy of Agricultural Sciences(NO.2017NZ04)

摘　　要：[Objectives] The aims were to optimize the extraction process of selenoproteins from selenium-enriched rice in Guangxi and provide references for the intensive processing and comprehensive utilization of selenium protein resources. [Methods]Selenium-enriched rice was used as materials to extract selenoproteins by phosphate buffer extraction method and to optimize the extraction process of selenoproteins by using the orthogonal experiment. Proteins and selenium content was measured by Coomassie Brilliant Blue G-250 reagent and AFS（ atomic fluorescence spectrometry） respectively. [Results] The most significant factor affecting extraction of rice Selenoproteins was extraction NaO H concentration,followed by the ratio of solid-liquid,temperature and then extraction time. The optimum extraction conditions of selenoproteins from rice were extraction temperature of 50 ℃,NaO H concentration of 0. 14 mol/L,extraction time of 5 h,and solid-liquid ratio of 1∶ 30. [Conclusions]The alkali extraction process optimized by orthogonal test could effectively improve the extraction rate of selenoproteins,and the optimized process parameters could be popularized and applied in practical production.[Objectives] The aims were to optimize the extraction process of selenoproteins from selenium-enriched rice in Guangxi and provide references for the intensive processing and comprehensive utilization of selenium protein resources. [Methods]Selenium-enriched rice was used as materials to extract selenoproteins by phosphate buffer extraction method and to optimize the extraction process of selenoproteins by using the orthogonal experiment. Proteins and selenium content was measured by Coomassie Brilliant Blue G-250 reagent and AFS（ atomic fluorescence spectrometry） respectively. [Results] The most significant factor affecting extraction of rice Selenoproteins was extraction NaO H concentration,followed by the ratio of solid-liquid,temperature and then extraction time. The optimum extraction conditions of selenoproteins from rice were extraction temperature of 50 ℃,NaO H concentration of 0. 14 mol/L,extraction time of 5 h,and solid-liquid ratio of 1∶ 30. [Conclusions]The alkali extraction process optimized by orthogonal test could effectively improve the extraction rate of selenoproteins,and the optimized process parameters could be popularized and applied in practical production.

关 键 词：Selenium-enriched rice SELENOPROTEINS Orthogonal experiment Extraction efficiency 

分 类 号：S511[农业科学—作物学]