呼肠孤病毒感染的树鼩中三类CD分子及IFN-γ表达变化及特征  

Characteristics and changes of the CD molecules and IFN-γ expressions in tree shrews infected with mammalian orthoreovirus

作　　者：袁圆 王璇 张志成 李娜[1] 王文广[1] 匡德宣[1] 代解杰[1] YUAN Yuan, WANG Xuan, ZHANG Zhicheng, LI Na, WANG Wenguang, KUANG Dexuan, DAI Jiejie(Center of Tree Shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medicine Sciences（CAMS） ＆ Peking Union Medical College （PUMC）. Yunnan Innovation Team of Standardization and ApplicationResearch in Tree Shrew. The Key Laboratory of Yunnan Province for Ophthalmic Research and Disease Control.Kunming 650118, China)

机构地区：[1]中国医学科学院/北京协和医学院医学生物学研究所中国医学科学院医学生物学研究所实验树鼩标准化与应用研究省创新团队云南省眼科疾病防治研究重点实验室,昆明650118

出　　处：《中国实验动物学报》2018年第3期323-330,共8页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家科技支撑计划(No.2014BAI01B00);云南省联合支持国家计划项目(No.2015GA009);云南省重大科技专项(No.2017ZF007)~~

摘　　要：目的探讨树鼩呼肠孤病毒激起机体免疫变化的特征,为树鼩的病毒防范提供理论基础。方法选取40~50日龄的树鼩分为三组(MRV1/TS/2011病毒株、MRV3/TS/2013病毒株和空白对照),通过灌胃后,采集第1、8、14、21、28天尾静脉血样,RT-PCR检测病毒载量、流式细胞术检测CD4/CD8/CD19、ELISA检测IFN-γ。结果感染第14天,MRV1/TS/2011和MRV3/TS/2013组的树鼩病毒载量、CD_4^+及CD19+细胞数量达峰值,其中经MRV1/TS/2011感染后第1天CD_4^+就有较高表达且数值与对照组相比差异有显著性,CD_8^+细胞数量及IFN-γ表达量在感染后第21天达到峰值。CD_4^+经MRV1/TS/2011感染后表达量更高,CD_8^+经MRV3/TS/2013感染后表达量更高。结论初步得出MRV1/TS/2011及MRV3/TS/2013感染机体后,随病毒载量变化,CD4/CD8/CD19及IFN-γ产生的变化规律:MRV1/TS/2011感染前期主要激起机体体液免疫,CD_4^+细胞发挥主要作用;MRV3/TS/2013可能主要影响机体细胞免疫,体液免疫只在病毒表达量显著升高及感染后期发挥作用,CD_8^+细胞和IFN-γ通过细胞免疫参与抗病毒反应。CD_4^+细胞可能对1型呼肠孤病毒更敏感,而CD19细胞+可能对3型呼肠孤病毒更敏感。对树鼩呼肠孤病毒的感染特征研究和树鼩本身的病毒防范有一定指导意义。Objective To explore the characteristics of immunological changes in tree shrews infected with orthoreovirus,and provide a theoretical basis for the prevention of virus in tree shrews. Methods 40-50-day-old tree shrews were divided into three groups： MRV1/TS/2011 virus-infected and MRV3/TS/2013 virus-infected groups,and saline-treated control group. On the 1,8,14,21,and 28 days after infection,blood samples were taken from the tail vein and used for RT-PCR,flow cytometry and ELISA detection,to assess the viral load,number of CD4/CD8/CD19 cells,andIFN-gamma expression. Results The MRV1/TS/2011 and MRV3/TS/2013 viral load in the plasma and the number of CD4^＋and CD19^＋cells reached a peak at the 14 th day after infection. At the first day after MRV1/TS/2011 infection,the CD4^＋cells had a significantly higher expression compared with the normal group. CD8^＋ cells and the IFN-gamma expression reached a peak at the 21 st day after infection. The expression of CD4^＋was even higher after MRV1/TS/2011 infection,and the expression of CD8^＋ cells was higher after MRV3/TS/2013 infection. Conclusions We would conclude that after MRV1/TS/2011 and MRV3/TS/2011 virus infection,accompanying the changes of viral load,it shows some regularity of the expression of CD4/CD8/CD19 and IFN-gamma in the tree shrews： at the early stage of MRV1/TS/2011 virus infection,humoral immunity is stimulated,and CD4^＋cells play a major role. MRV3/TS/2013 virus may mainly affect the cellular immunity,while humoral immunity only plays a role at a high viral expression or the late stage of infection. CD4^＋cells may be more sensitive to type 1 reovirus,and CD19＋cells may be more sensitive to type 3 reovirus.

关 键 词：树鼩呼肠孤病毒 免疫应答 流式细胞术 CD分子 细胞因子 

分 类 号：Q95-33[生物学—动物学]