F-box基因FOF2在拟南芥盐和冷胁迫响应中的功能分析  

Function Analysis on F-box Gene FOF2 Response to Salt Stress and Cold Stress in Arabidopsis

作　　者：刘选明 孙孟思 李新梅 贺热情 屈丽娜 唐冬英 赵小英 LIU Xuanming;SUN Mengsi;LI Xinmei;HE Reqing;QU Lina;TONG Dongying;ZHAO Xiaoying(Hunan Province Key Laboratory of Plant Function Genomics and Developmental Regulation,College of Biology,Hunan University,Changsha 410082,China)

机构地区：[1]湖南大学生物学院植物功能基因组学与发育调控湖南省重点实验室,湖南长沙410082

出　　处：《湖南大学学报（自然科学版）》2018年第6期120-127,共8页Journal of Hunan University:Natural Sciences

基　　金：国家自然科学基金资助项目(31171176);湖南省生物发育工程与新产品研发协同创新中心项目(20134486)~~

摘　　要：FOF2为F-box蛋白家族成员,其生物学功能尚不清楚.采用实时荧光定量PCR和生理学实验相结合的方法,对FOF2基因的表达模式及其在拟南芥抗盐和冷胁迫响应中的作用进行了分析.研究发现,FOF2在拟南芥根、茎生叶和果荚中表达较高,并且其表达受盐和冷胁迫诱导.FOF2过表达株系对盐胁迫敏感,与野生型相比种子萌发率低、幼苗主根较短;相反,fof2突变体对盐胁迫的敏感性则减弱.FOF2过表达和缺失突变体种子萌发对冷胁迫无响应,但其主根在冷处理中分别比野生型短或者长.盐处理下,FOF2过表达株系中盐胁迫反应相关基因的表达量显著降低,fof2突变体中则升高;冷处理下,FOF2过表达株系中冷胁迫反应相关基因的表达量显著升高,fof2突变体中则降低.结果表明,FOF2在植物抗盐胁迫响应中起负调控作用,在抗冷胁迫响应中则可能起正调控作用.FOF2 is one of the F-box protein family members and its biological function remains unclear. The expression patterns and function of FOF2 in response to salt and cold stress in Arabidopsis were analyzed by real-time quantitative PCR and physiological method. The results showed that FOF2 was highly expressed in root, cauline leaf and silique in Arabidopsis, and its transcriptional level was induced by salt and cold stress. The seed germination and root elongation of FOF2 overexpression lines were hypersensitive to salt stress, while the fof2 mutant showed reduced sensitivity to salt stress compared with wild type. The seed germination of both the FOF2 overexpression lines and fof2 mutant showed no phenotype in response to cold, but their primary root length was shorter or longer than the wild type plant during cold stress, respectively. The salt stress responsive marker genes were down-regulated in FOF2 overexpression lines, but up-regulated in fof2 mutant under salt stress. The cold stress responsive marker genes were up-regulated in FOF2 overexpression lines, but down-regulated in fof2 mutant under cold stress. These results indicate that the FOF2 plays a negative role in salt tolerance, but positive role in cold tolerance in Arabidopsis.

关 键 词：功能分析 FOF2 F-box基因 逆境胁迫 拟南芥 

分 类 号：Q94[生物学—植物学]