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作 者:王传旗[1] 梁莎[1] 张文静 邓时梅 包赛很那 苗彦军[1] Wang Chuan-qi;Liang Sha;Zhang Wen-jing;Deng Shi-mei;Baosaihenna;Miao Yan-jun(Tibet Agricultural and Animal Husbandry University,Nyingchi 860000,Tibet,Chin)
机构地区:[1]西藏农牧学院,西藏林芝860000
出 处:《草业科学》2018年第6期1459-1464,共6页Pratacultural Science
基 金:国家重点研发计划项目(2016YFC0502002;2016YFC0502006);西藏自治区科技厅重点项目(2017ZDKJZC-15)
摘 要:利用聚乙二醇(PEG)试剂室内模拟不同干旱(0、-0.10、-0.20、-0.40、-0.80、-1.60 MPa)和温度(10、15、20、25、30℃)环境条件,对西藏班戈县野生型赖草(Leymus secalinus)种子萌发特性进行研究。结果表明,赖草种子在恒温10~30℃和变温10~30℃均能萌发.恒温25℃处理下,种子萌发达到了最佳响应;恒温15℃和变温20/30℃处理对赖草种子发芽率具有显著影响(P<0.05),而恒温10和30℃对赖草种子发芽率具有极显著影响(P<0.05);随着PEG渗透势的增加,赖草种子发芽率、发芽指数、活力指数和胚芽长均出现了不同程度的下降现象,而胚根则表现为先升后降的变化现象。25℃和蒸馏水处理下,野生赖草种子萌发效果最佳;而在25℃和-0.10~0 MPa渗透势处理下,赖草种子胚根优于胚芽生长。This study used a polyethylene glycol reagent and indoor simulation of different levels of drought stress(0,-0.10,-0.20,-0.40,-0.80,and-1.60 MPa)and temperatures(10,15,20,25,and 30℃)to study the seed germination characteristics of Leymussecalinusfrom Baingoin County of Xizang.The results showed that L.secalinus seeds can germinate in constant or variable(alternating)temperatures in the range of10~30 ℃.A constant temperature of 15 ℃ and alternating temperatures of 20/30 ℃ had significant effects on seed germination,while constant temperatures 10℃and 30℃ had extremely significant effects on germination percentage;seed germination was best at 25 ℃.With increasing PEG osmotic potential,the germination rate,germination index,vigor index,and plumule length showed different downward trends,while the radicle length first increased and then decreased.The germination rate of wild L.secalinus was the best(84%)under culture conditions involving 25 ℃ temperature and distilled water,among all the different temperatures and PEG osmotic potentials.At 25 ℃ and-0.10 to 0 MPa osmotic potential,the radicle growth of L.secalinus was better than that of the plumule.
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