绞股蓝总皂甙对氧化修饰低密度脂蛋白损伤小牛主动脉内皮细胞的防治作用  

Protective effects of gypenoside on cultured bovine aortic endothelial cells against injury induced by oxidatively modified low density lipoprotein

作　　者：许士凯[1] 何红[2] 王福俤[3] 

机构地区：[1]同济大学医学院药理学教研室,上海200331 [2]上海第二医科大学卫生事业管理系,上海200025 [3]第二军医大学海军卫生教研室,上海200433

出　　处：《中国临床药理学与治疗学》2002年第5期389-392,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的 :探讨绞股蓝总皂甙 (GYP)对氧化修饰低密度脂蛋白 (oxidativelymodifiedlowdensitylipopro tein ,oxLDL)损伤小牛主动脉内皮细胞的防治作用及可能机制。方法 :在体外培养的小牛主动脉内皮细胞 (bovineaorticendothelialcell ,BAEC )中加入oxLDL(终浓度为 0 .1gpr·L- 1)及GYP低、中、高剂量组 (5 .0 ,10 .0 ,2 0 .0mg·L- 1) ,培养 2 4h后 ,采用细胞毒试验、丙二醛 (MDA)测定及细胞计数等方法 ,分别对其贴壁细胞及培养液进行检测。结果 :①细胞毒试验发现 ,oxLDL对BAEC的生长增殖有明显的抑制作用 (2 0 .6 3% ) ,GYP组 (10 .0 ,2 0 .0mg·L- 1)为9.14 %和 6 .5 6 % (P <0 .0 1) ;②加oxLDL组和GYP组BAEC粘附的HL6 0细胞数明显多于对照组 (P <0 .0 1) ;③MDA检测发现oxLDL促进BAEC脂质过氧化物生成 ,并高于GYP组和对照组 (P <0 .0 1)。结论 :绞股蓝总皂甙具有对氧化修饰低密度脂蛋白损伤小牛主动脉内皮细胞的防治作用。AIM: To study the protective effects of gypenoside (GYP) on cultured bovine aortic endothelial cells against injury induced by oxidatively modified low density lipoprotein (oxLDL). METHODS: Bovine aortic endothelial cells (BAEC) were cultured and treated with oxLDL ( 0.1 g pr·L -1 ) and the different concentrations of GYP ( 5.0 , 10.0 , and 20.0 mg·L -1 ) for 24 h. The cultured cells and the contents in the medium were determined by MTT colorimetric assay, TBA method,cell calculating and so on.RESULTS: The inhibitory effects were shown in the proliferation of BAEC by oxLDL ( 20.63 %) and GYP ( 10.0 and 20.0 mg·L -1 ) ( 9.14 % and 6.56 %) (P< 0.01 ). The number of HL60 monocyte cells adhered to BAEC in oxLDL group and GYP group was markedly higher than that in control group (P< 0.01 ). MDA content in culture media in oxLDL group was significantly higher than that in control group and GYP group (P< 0.01 ). CONCLUSION: GYP can protect cultured bovine aortic endothelial cells from injury induced by oxidatively modified low density lipoprotein.

关 键 词：绞股蓝总皂甙 内皮细胞 氧化低密度脂蛋白 细胞增殖 脂质过氧化 主动脉 丙二醛 动脉粥样硬化 细胞毒试验 AS 

分 类 号：R543.5[医药卫生—心血管疾病] R965[医药卫生—内科学]