丝状真菌F54的分离鉴定及其对铯的吸附特性  

Isolation and Identification of Filamentous Fungi F54 and Its Biosorption and Enrichment Characteristics of Cesium

作　　者：张丽娟[1,2] 王玮 张志东[1,2] 谢玉清 顾美英[1,2] 朱静 唐琦勇[1,2] 王博 宋素琴[1,2] 黄伟 ZHANG Li juan1,2 , WANG Wei1,2, ZHANG Zhi dong1,2 , XIE Yu qing1,2 ,GU Mei ying1,2 , ZHU Jing1,2 , TANG Qi yong1,2 , WANG Bo1,2, SONG Su qin1,2 , HUANG Wei1,2(1. Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China; 2. Xinjiang Laboratory of Special Enviromental Microbiology, Urumqi 830091, Chin)

机构地区：[1]新疆农业科学院微生物应用研究所,新疆乌鲁木齐830091 [2]新疆特殊环境微生物实验室,新疆乌鲁木齐830091

出　　处：《核化学与放射化学》2018年第3期175-182,共8页Journal of Nuclear and Radiochemistry

基　　金：国家"863"项目(No.2012AA063503);国家自然科学基金资助项目(No.31560034;No.31560023);新疆维吾尔自治区"万人计划"后备人选培养项目(No.wr2015cx07);新疆自治区自然基金资助项目(No.2016D01A050)

摘　　要：从新疆放射性污染土壤样本中,采用微生物分离方法,结合压力试验及吸附实验,获得一株对放射性核素污染具有修复能力的土著丝状真菌F54。通过形态学、分子生物学鉴定及Biolog碳源利用实验,F54为镰刀霉菌属真菌,与Fusarium oxysporum strain ATCC MYA-3931同源性最高。最适宜生长条件是:马铃薯葡萄糖培养基,30℃,pH=7.0。菌株F54吸附Cs+的最佳条件是:pH=7.0,30℃,伴随生长进行吸附约3~4d,吸附率最高能达到约84%,常见金属阳离子K^+、Na^+、Ca^(2+)、Mg^(2+)、Al^(3+)、Fe^(3+)对其吸附均有一定的抑制作用。菌株F54能够耐受10kGy的钴源照射,对重金属离子Ni^(2+)、Cr^(3+)、Zn^(2+)、Co^(2+)、Pb^(2+)、Hg^(2+)均有较高耐受性,对放射性137 Cs的吸附率为62%。菌株F54在有较高的重金属离子混合污染的放射性环境中具有一定的修复应用潜能。In order to obtain indigenous strains with radionuclide remediation ability in radioactive pollution area in Xinjiang,soil samples were collected for separation of microorganisms.After the pressure tests and adsorption experiments,a filamentous fungus F54 was finally obtained.Through the traditional morphology and molecular biology identification,strain F54 belongs to Fusarium sp.,exhibits the highest homology with Fusarium oxysporum strain ATCC MYA-3931.The optimum growth condition is：potato dextrose medium,pH=7.0.The optimum absorption conditions of Cs＋for F54 strain are：pH=7.0,30 ℃,3-4 dassociated with the growth.The Cs＋adsorption efficiency of F54 can up to be about84%tested by ICP-MS.Common cations such as K^＋,Na^＋,Ca^2＋,Mg^2＋,Al^3＋,Fe^3＋can inhibit the adsorption of Cs＋.The F54 strain can tolerate 10 kGy dose irradiation of radioactive cobalt,and also has high tolerance of heavy mental ions Ni^2＋,Cr^3＋,Zn^2＋,Co^2＋,Pb^2＋,Hg^2＋.The adsorption efficiency of radioactive 137 Cs can up to be 62%tested by liquid scintillator.Strain F54 has high potential to repair radioactive pollution in heavy metal contaminated environment.

关 键 词：铯 丝状真菌 生物吸附 

分 类 号：TL732[核科学技术—辐射防护及环境保护]