运动训练对背根神经节切除模型大鼠运动功能的影响  

Effect of treadmill training on the locomotor function in a rat model of dorsal root ganglion resection

作　　者：安普天 朱文文 李脉超 崔晓娟 周燕 张益萌 金利新[2] An Pu-tian1, Zhu Wen-wen1, Li Mai-chao1, Cui Xiao-juan1, Zhou Yan1, Zhang Yi-meng1, Jin Li-xin2(1.School of Clinical Medicine, 2.Department of Anatomy, Medical College of Qingdao University, Qingdao 266071, Shandong Province, Chin)

机构地区：[1]青岛大学医学部临床医学院,山东省青岛市266071 [2]青岛大学医学解剖学教研室,山东省青岛市266071

出　　处：《中国组织工程研究》2018年第16期2537-2541,共5页Chinese Journal of Tissue Engineering Research

基　　金：青岛大学医学院国家级实验教学示范中心创新实验项目(201511065055)

摘　　要：背景:周围神经损伤后可导致中枢神经系统通路发生广泛的变化,运动训练对于中枢神经系统损伤后运动功能恢复有一定的促进作用。目的:观察运动训练后部分感觉缺失模型大鼠的运动功能变化及相应脊髓节段囊泡谷氨酸转运蛋白表达的变化规律,探究运动训练对背根神经节切除模型大鼠运动功能的影响。方法:10周龄雄性Wistar大鼠39只随机分成实验组(15只)、对照组(15只)和假手术组(9只)。实验组和对照组切除右侧L3、L4背根神经节制备局部感觉缺失的周围神经损伤模型,假手术组只咬除相应关节突,分离至观察到背根神经节。实验组于造模后7 d开始进行15 m/min的跑步机训练,对照组和假手术组自由活动。在术前3 d及术后7,14,21,28 d进行大鼠步态分析,观察模型大鼠行为学变化。于术后7,14,28 d取大鼠L3脊髓节段制作组织切片,免疫组织化学观察脊髓内囊泡谷氨酸转运蛋白1变化及其特点。结果与结论:(1)步态分析:实验组、对照组造模后各时间点腓神经功能指数值均低于术前(P<0.05)和假手术组(P<0.05);实验组、对照组造模后7 d腓神经功能指数值最低(P<0.05),随后逐渐提高,但仍低于术前水平(P<0.05);实验组造模后21及28 d腓神经功能指数值显著大于对照组(P<0.05);(2)脊髓内囊泡谷氨酸转运蛋白1表达:实验组、对照组造模后各时间点脊髓第IX板层囊泡谷氨酸转运蛋白1均明显低于假手术组(P<0.01);实验组造模后14,28 d脊髓第IX板层囊泡谷氨酸转运蛋白1高于对照组(P<0.05);实验组与对照组术后各时间点脊髓第IX板层囊泡谷氨酸转运蛋白1表达呈下降趋势(P<0.05),对照组下降更加明显;(3)结果表明:运动训练可以促进周围神经损伤后运动功能的恢复。BACKGROUND： Peripheral nerve injury can lead to extensive changes in central nervous system, and exercise training can promote the recovery of locomotor function following central nervous system injury. OBJECTIVE： To observe the changes of locomotor function and the expression levels of vesicular glutamate transporter VGLUT1 in the spinal cord in a rat model of dorsal root ganglion resection after treadmill exercise and to explore the effect of treadmill training on the locomotor function after peripheral nerve injury. METHODS： Thirty-nine 10-week-old male Wistar rats were randomized into experimental（n=15）, control（n=15） and sham operation（n=9） groups. The rats in the experimental and control groups received the dorsal root ganglion resection at L3 and L4 segments to establish the model of peripheral nerve injury under local anesthesia, while the rats in the sham operation group were only subjected to dorsal root ganglion exposure. The rats in the experimental group underwent 15 m/minute treadmill training at postoperative 7 days, while rats in the other two groups were in free movement. Gait analysis was performed at preoperative 3 days, postoperative 7, 14, 21, and 28 days, respectively, and the behavioral changes of rats were observed. The tissue sections were obtained from L3 segment at postoperative 7, 14, and 28 days to detect the expression levels of VGLUT1 in the spinal cord by immunohistochemistry. RESULTS AND CONCLUSION： The peroneal nerve function index in the experimental and control groups was lower than that before surgery and that in the sham operation group at postoperative different time points（all P〈0.5）. The index in the experimental and control groups was the lowest on day 7 postoperatively（P〈0.5）, then the index gradually increased, but was still lower than the preoperative level（P〈0.5）. The index in the experimental group was significantly higher than that in the control group at postoperative 21 and 28 days（P〈0.5）. The expression levels of VGLUT1

关 键 词：囊泡谷氨酸转运蛋白类 周围神经 康复 脊神经后根神经节切除术 运动训练 步态分析 囊泡谷氨酸转运蛋白 周围神经损伤 运动康复 WISTAR大鼠 腓神经功能指数 

分 类 号：R318[医药卫生—生物医学工程]