湿地松×洪都拉斯加勒比松遗传多样性的ISSR分析（英文）  

Genetic diversity analysis of Pinus elliottii × P. caribaea var. hondurensis using ISSR markers

作　　者：李义良 赵奋成[1,2] 李福明 钟岁英[3] 谭志强 吴惠姗 郭文冰 廖仿炎 LI Yiliang;ZHAO Fencheng;LI Fuming;ZHONG Suiying;TAN Zhiqiang;WU Huishan;GUO Wenbing;LIAO Fangyan(Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization,Guangzhou 510520,China;Guangdong Academyof Forestry,Guangzhou 510520,China;Taishan Hongling Seed Orchard,Taishan 529200,Guangdong,China)

机构地区：[1]广东省森林培育与保护利用重点实验室,广州510520 [2]广东省林业科学研究院,广州510520 [3]台山市红岭种子园,广东台山529200

出　　处：《广西植物》2018年第6期812-817,共6页Guihaia

基　　金：广东省林业科技创新专项项目(2016KJCX021)~~

摘　　要：杂交育种是产生遗传变异、表型变异及选择新变异的重要方法。然而系统发育不清晰,选择较近的亲缘关系亲本用于杂交子代往往表现出较低的遗传多样性。为探究湿地松、洪都拉斯加勒比松种间杂交后代遗传多样性水平,对8个湿地松×洪都拉斯加勒比松家系进行ISSR分析。利用10条引物共产生60个表达清晰可用于分析的标记,其中48个标记表现为多态性,占总标记数的80%;湿加松各个家系多态位点百分率在5%～23.33%之间;各个家系基因多样性指数在0.015 2～0.087 2之间,Shannon指数的范围在0.021 6～0.129 4之间(家系水平为0.293 4)。8个家系间的基因分化系数Gst为0.743 5,即总的遗传变异中有74.35%的变异存在于家系间,家系内的遗传变异占总遗传变异的25.65%。采用UPGMA法对湿加松的8个家系进行了聚类分析,确定了各个家系之间的遗传亲缘关系。8个家系间的基因流N_m为0.172 5,表明基因流处于较低水平。Cross-breeding is an important method for generating genetic and phenotypic variation for selecting new varie-ties. However, because of an uncertainty of phylogenetic relationships, the parents selected for crosses may have a closegenetic relationship resulting in hybrid progeny that shows low genetic diversity. Analysis of inter-family genetic diversitywas undertaken among eight Pinus elliottii × P. caribaea var. hondurensis full-sib families using inter-sequence simple re-peat （ISSR） markers. A total of 480 individuals were analyzed using 10 ISSR primers. Nei’s unbiased gene diversity inthe families ranged from 0.015 2 to 0.087 2. Shannon genetic diversity index values ranged from 0.021 6 to 0.129 4. Onlya small proportion （25. 65%） of genetic variation resided within families, whereas the majority of genetic variation（74.35%） accounted for the inter-family genetic differentiation index of Gst= 0.743 5. On the basis of estimated geneticdistance and UPGMA clustering analysis, the genetic differentiation among the eight families was indicated to be rela-tively high with low gene flow （Nm = 0.172 5）. The low inter-family gene flow may be related to the high genetic heterozygosity of slash pine and Caribbean pine. These findings are expected to provide a foundation for genetic breeding ofPinus elliottii × P. caribaea var. hondurensis hybrids.

关 键 词：湿加松 家系 遗传多样性 ISSR标记 

分 类 号：Q949[生物学—植物学]