七种风铃草属植物亲缘关系的ISSR分析  

ISSR analysis on genetic relationships among seven species in Campanula

作　　者：包志远 曲继鹏[3] 杨在君[1] 路璐[4] 廖明莉[1] 周梦霞 彭正松[1,3] BAO Zhiyuan;QU Jipeng;YANG Zaijun;LU Lu;LIAO Mingli;ZHOU Mengxia;PENG Zhengsong(Key Laboratory of Southwest China Wildlife Resources Conservation / Ministry of Education,China West Normal University,Nanchong 637009,Sichuan,China;School of Agricultural Sciences,Xichang College,Xichang 615013,Sichuan,China;ZhejiangJianyuan Architectural Design and Urban Planning Institute,Hangzhou 310004,China;College of EnvironmentalScience and Engineering,China West Normal University,Nanchong 637009,Sichuan,China)

机构地区：[1]西华师范大学西南野生动植物资源保护教育部重点实验室,四川南充637009 [2]浙江建院建筑规划设计院,杭州310004 [3]西昌学院农业科学学院,四川西昌615013 [4]西华师范大学环境科学与环境工程学院,四川南充637009

出　　处：《广西植物》2018年第6期818-824,共7页Guihaia

基　　金：四川省科技厅应用基础项目(16JC0022);西华师范大学英才基金(17YC355)~~

摘　　要：为了解四川风铃草属植物的资源丰富度和遗传进化情况,该研究利用ISSR分子标记,探究了四川7种风铃草属植物的亲缘关系,为风铃草属植物的分子标记辅助鉴定、资源保护、花卉品种的开发与育种提供理论基础。结果表明:40条ISSR引物中有28条引物能够扩增出清晰的条带,扩增总条带数为164,其中有98.8%的扩增条带具有多态性,供试的7种风铃草属植物遗传相似性系数在0.421～0.945之间,其中钻裂风铃草与藏滇风铃草的遗传相似度最高为0.945,说明它们之间的亲缘关系很近。此外,ISSR分子标记聚类结果表明,7种风铃草属植物可以明显聚为4大类:西南风铃草、灰毛风铃草、灰岩风铃草为一类;紫斑风铃草与流石风铃草各成一类,这两种在形态上与其它各种风铃草差异较大;最后是钻裂风铃草与藏滇风铃草为一类。Genetic relationships among seven species in Campanula L. were investigated by inter-simple sequence repeatmolecular markers （ISSR）. The results showed that 28 of 40 primers can be amplified to clear bands. All the 28 ISSRprimers amplified 164 bands, and the proportion of polymorphic fragment was 98.8%. The value of genetic similarity（GS） indexes of seven species based on the ISSR data varied from 0.421 to 0.945, and the highest genetic similarity was0.945 between C. aristata and C. modesta, which indicated that they had closely relative relationship. Cluster analysisshowed that all the species could be divided into four groups： C. colorata, C. cana and C. calcicola in the first group,C. calcicola in the second group, C. crenulata in the third group, and C. aristata and C. modesta in the last group. Thisstudy provides reference for the molecular marker assisted identification, resource conservation, flower variety develop-ment and breeding of Campanula.

关 键 词：风铃草属 ISSR分子标记 遗传多样性 

分 类 号：Q948[生物学—植物学]