食管癌中miR-203和miR-21的表达水平及临床意义  

miR-203 and miR-21 expression levels in esophageal carcinoma and their clinical significance

作　　者：陈剑峰[1] 沈宗坤[1] CHEN Jianfeng;SHEN Zongkun(De partment of Clinical Laboratory,Fuxin Central Hospital,Fuxin,Liaoning 123000,China)

机构地区：[1]辽宁省阜新市中心医院检验科,123000

出　　处：《检验医学与临床》2018年第12期1770-1773,共4页Laboratory Medicine and Clinic

摘　　要：目的探讨食管癌组织中miR-203和miR-21的表达水平及其临床意义。方法收集2011年9月至2013年9月该院病理科47例食管癌患者的肿瘤组织标本及相应癌旁正常组织标本,采用荧光定量聚合酶链反应检测两种组织中miR-203、miR-21的表达,分析miR-203、miR-21表达与食管癌组织病理特征及患者预后的关系。结果食管癌组织中miR-203表达水平为0.35±0.21,明显低于癌旁正常组织的0.87±0.14,miR-21表达水平为1.37±0.23,明显高于癌旁正常组织的0.79±0.17,差异均有统计学意义(P<0.05);miR-203表达与食管癌临床分期、肿瘤分化程度、淋巴结转移密切相关(t/F=5.629、15.021、3.159,P=0.000、0.000、0.003),而与食管癌患者年龄、性别、肿瘤部位无明显相关性(t=0.321、0.776、0.498,P=0.750、0.442、0.621);miR-21表达与食管癌患者临床分期、肿瘤分化程度、淋巴结转移密切相关(t/F=4.201、41.646、5.109,P=0.000、0.000、0.000),而与食管癌患者年龄、性别、肿瘤部位无明显相关性(t=0.732、1.436、0.597,P=0.468、0.158、0.553)。miR-203高表达组生存率明显高于miR-203低表达组,差异有统计学意义(P<0.05);miR-21低表达组生存率明显高于miR-21高表达组,差异有统计学意义(P<0.05)。Spearman秩和检验结果显示,食管癌组织中miR-203与miR-21表达呈明显负相关(r=-0.564,P<0.05)。结论 miR-203在食管癌组织中明显低表达,miR-21为明显高表达,二者有望成为食管癌早期诊断及预后判断的有效标志物。Objective To explore miR-203 and miR-21 expression levels in esophageal carcinoma and their clinical significance.Methods A total of 47 specimens esophageal carcinoma tissue and corresponding para normal tissue specimens in Department of Clinical Pathology of the hospital from September 2011 to September 2013 were collected as the study objects.miR-203 and miR-21 expression levels in tissues were detected by using quantitative fluorescence PCR（qRT-PCR）method.The relationship between miR-203 and miR-21 expression and pathological features and the prognosis of esophageal carcinoma were analyzed.Results miR-203 expression level was 0.35±0.21 in esophageal cancer tissues,which was significantly lower than 0.87±0.14 in normal tissues,and the difference was statistically significant（P〈0.05）.miR-21 expression level in esophageal carcinoma was 1.37±0.23,which was significantly higher than 0.79±0.17 in the normal tissues,and the difference was statistically significant（P〈0.05）.miR-203 expression was closely related to clinical stage,tumor differentiation and lymph node metastasis of patients with esophageal carcinoma（t/F=5.629,15.021,3.159,P=0.000,0.000,0.003）,and not significantly related to age,sex and tumor location of patients with esophageal carcinoma（t=0.321,0.776,0.498,P=0.750,0.442,0.621）.miR-21 expression level was closely related to clinical stage,tumor differentiation and lymph node metastasis of patients with esophageal carcinoma（t/F=4.201,41.646,5.109,P=0.000）,and not significantly related to age,sex and tumor location of patients with esophageal carcinoma（t=0.732,1.436,0.597,P=0.468,0.158,0.553）.Survival rate of miR-203 high expression group was significantly higher than that in miR-203 low expression group,and the difference was statistically significant（P〈0.05）.Survival rate of miR-21 low expression group was higher than that of miR-21 high expression group,and the difference was statistically significant（P〈0.05）.The results of Spearman test showed that miR-2

关 键 词：食管癌 miR-203 MIR-21 病理特征 预后 

分 类 号：R563[医药卫生—呼吸系统]