江苏省吕四港潮间带贝类的蛋白质组分析(英文)  

Proteomic Profiling in Shellfish From Lüsi Aquaculture in the Intertidal Zone Area of the Jiangsu Province

作　　者：盛乃娟 王倩[1] 吴体智 陈晓钰[1] 李晓芳[1] 刘睿[1] 吴皓[1] SHENG Nai-juan;WANG Qian;WU Ti-zhi;CHEN Xiao-yu;LI Xiao-fang;LIU Rui;WU Hao(Jiangsu Key Laboratory of Research and Development in Marine Bio resource Pharmaceutics,Jiangsu Collaborative Inno ration Center of Chinese Medicinal Resources Industrialization,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine,Nanjing,210023,China)

机构地区：[1]南京中医药大学,江苏省海洋药用生物资源研究与开发重点实验,江苏省中药资源产业化过程协同创新中心,中药资源产业化与方剂创新药物国家地方联合工程研究中心,江苏南京210028

出　　处：《南京中医药大学学报》2018年第3期222-226,共5页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家海洋公益性行业科研专项(201305007,201405017); 国家高新技术研究发展计划(“863”计划)项目(2013AA093003); 江苏高校优势学科建设工程资助项目(PAPD); 江苏省海洋科技创新专项(HY2017-7); 药用南珠标准化养殖与综合利用产业链协同创新(Bhsfs002); 江苏省高等学校自然科学研究重大项目(17KJA360005); 江苏省高校“青蓝工程”优秀青年骨干教师(2016年); 江苏省“333工程”第三层次(2016年)资助

摘　　要：潮间带滩涂是江苏省海岸线的重要特征,腹足类、双壳贝类海洋生物资源丰富。该文基于shotgun蛋白质鉴定技术研究江苏省吕四港地区主要贝类的蛋白质组成。采用nano-LC MS/MS技术从9种贝类生物中共鉴定了265个蛋白质,其中角螺中鉴定的蛋白质最多(105个),从毛蚶中鉴定的蛋白质最少(39个)。这些蛋白质以细胞骨架蛋白为主,如微管蛋白、肌动蛋白、原肌球蛋白、肌球蛋白重链及组蛋白;另外还鉴定出核糖体蛋白、精氨酸激酶、ATP合成酶、延伸因子、热休克蛋白等。采用PCA分析法将蛋白质按照细胞成分与分子功能进行统计分析,结果表明缢蛏、泥螺与角螺的蛋白质组成相似,而竹蛏、文蛤、杂色蛤和四角蛤蜊的蛋白质组成相似。Heatmap和聚类分析结果表明,杂色蛤和西施舌被聚为一类,而缢蛏、泥螺、角螺、文蛤、毛蚶和四角蛤蜊被聚为另一类,竹蛏与这两类距离较远。该文研究建立了江苏沿海潮间带主要贝类中蛋白质组成特点比较分析的方法,有利于江苏沿海贝类资源开发利用。Jiangsu province is rich in intertidal marine resources,especially gastropod and bivalve shellfish.In this study,we performed shotgun proteomic analysis on shellfish from Lüsi,Jiangsu to identify protein content.In total,265 proteins were identified in nine species of shellfish using nano LC-MS/MS.The highest number of proteins were identified in the gastropod Hemifusus tuba（HF）（105 proteins）,whereas the bivalve Scapharca subcrenata（SSC）produced the fewest（39 proteins）.The most abundant proteins are constituents of cytoskeleton,including actin,tubulin,tropomyosin,myosin heavy chain,as well as histone proteins.Additional proteins were also identified,including ribosomal proteins,enzymes such as arginine kinase and ATP synthase,and housekeeping proteins such as elongation factor and heat shock protein.Proteins were classified based on cellular components and molecular function using the principal component analysis（PCA）.Results of PCA analysis revealed that Sinonovacula constrzcta（SC）,Bullacta exarata（BE）and Hemifusus tuba（HF）showed similar protein profiles,whileSolen strictus（SS）,Meretrix meretrix（MM）,Ruditapes philippinarum（RP）,and Mactra veneriformis（MV）also showed similar protein profiles.Hierarchical clustering results of heat map studies showed that RP and Mactra antiquate（MA）were classified into one group.SC,BE,HF,MM,SSC,and MV were classified into another group.SS showed the furthest distance from this group.We established a method for analyzing and characterizing the protein composition characteristics of predominant shellfish species in Jiangsu providence＇s intertidal zone area,which provides basic data for the development of shellfish resources.

关 键 词：蛋白质组 贝类 江苏 主成分分析 潮间带 

分 类 号：R282.77[医药卫生—中药学]