牙科修复材料苯扎氯铵表面活性剂对5种常见口腔致病菌的体外抑菌效果  

In vitro antibacterial effect of benzalkonium chloride on five common oral pathogens

作　　者：代静[1] 陈燕斌[1] 陈珊[1] 任静[1] 李昆蔓[1] 杨军英[1] Dai Jing;Chen Yan-bin;Chen Shan;Ren Jing;Li Kun-man;Yang Jun-ying(Department of Stomatology,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,Guangdong Province,China)

机构地区：[1]中山大学附属第一医院口腔科,广东省广州市510080

出　　处：《中国组织工程研究》2018年第18期2849-2854,共6页Chinese Journal of Tissue Engineering Research

基　　金：横向课题(K0601161):苯扎氯铵对口腔疾病常见致病菌的体外抑菌实验~~

摘　　要：背景:目前,苯扎氯铵已被用于牙科修复材料中,能增强材料的抗菌性并作用持久。然而,目前尚缺乏苯扎氯铵与牙髓牙周感染相关细菌作用的研究。目的:研究苯扎氯铵对口腔主要致病菌的体外抑菌效果。方法:采用琼脂扩散法比较0.1%苯扎氯铵、体积分数3%双氧水、生理盐水与5.25%次氯酸钠对牙龈卟啉单胞菌、中间普氏菌、伴放线放线杆菌、变形链球菌、粪肠球菌的体外抑菌效果,采用试管稀释法测定苯扎氯铵对上述细菌的最低抑菌浓度。结果与结论:(1)对牙龈卟啉单胞菌与中间普氏菌,0.1%苯扎氯铵表现出了较强的抑菌效果,抑菌效果弱于5.25%次氯酸钠(P<0.05);对牙龈卟啉单胞菌,0.1%苯扎氯铵的抑菌效果与体积分数3%双氧水无差异(P>0.05);对中间普氏菌,0.1%苯扎氯铵的抑菌效果优于体积分数3%双氧水(P<0.05);对伴放线放线杆菌和变形链球菌,0.1%苯扎氯铵的抑菌效果均优于体积分数3%双氧水(P<0.05),与5.25%次氯酸钠无差异(P>0.05);对粪肠球菌,0.1%苯扎氯铵的抑菌效果优于体积分数3%双氧水(P<0.05),弱于5.25%次氯酸钠(P<0.05);体积分数3%双氧水对粪肠球菌不产生抑菌环;(2)0.1%苯扎氯铵对牙龈卟啉单胞菌、中间普氏菌、伴放线放线杆菌、变形链球菌、粪肠球菌的最低抑菌浓度分别为16,2,4,2,4 mg/L;(3)结果表明,0.1%苯扎氯铵对牙龈卟啉单胞菌、中间普氏菌、伴放线放线杆菌、变形链球菌、粪肠球菌具有较强的抑菌效果。BACKGROUND： Benzalkonium chloride has been used in dental restorative materials to enhance the long-lasting antibacterial properties of materials. OBJECTIVE： To evaluate the antibacterial activity of benzalkonium chloride on oral common pathogenic bacteria in vitro. METHODS： The agar diffusion method was used to determine the inhibitory effects of antibacterial agents, 0.1% benzalkonium chloride, 3% H2O2and 5.25% sodium hypochlorite, on five kind of oral pathogens, Porphyromonas gingivalis （P.g）, Prevotella intermedia （P.i）, Actinobacillus actionmycemcomitans （A.a）, Streptococcus mutans （S.m） and Enterococcus faecalis （E.f）. The tube dilution method was used to detect the minimal inhibitory concentration of benzalkonium chloride against the five bacteria mentioned above. RESULTS AND CONCLUSION： The antibacterial effect of 0.1% benzalkonium chloride on P.g was not significantly different from that of 3% hydrogen peroxide （P〉0.05）, while 0.1% benzalkonium chloride showed better effect on P.i than 3% hydrogen peroxide （P〈0.05）. On P.g and P.i, the antibacterial effect of 0.1% benzalkonium chloride was worse than that of 5.25% sodium hypochloritethe （ P〈0.05）. The antibacterial effect of 0.1% benzalkonium chloride on A.a and S.m was better than that of 3% hydrogen peroxide （P〈0.05）, and similar to that of 5.25% sodium hypochlorite （P〉0.05）. The antibacterial effect of 0.1% benzalkonium chloride on E.f was better than that of 3% hydrogen peroxide （P 〈 0.05）, but worse than that of 5.25% sodium hypochlorite （P 〈 0.05）. The minimal inhibitory concentration of benzalkonium chloride to P.g, P.i, A.a, S.m, E.f was 16, 2, 4, 2, 4 mg/L, respectively. To conclude, 0.1% benzalkonium chloride has strong antibacterial effects on P.g, P.i, A.a, S.m and E.f.

关 键 词：口腔材料 苯扎氯铵溶液 牙龈卟啉单胞菌 中间普氏菌 伴放线放线杆菌 变形链球菌 粪肠球菌 琼脂扩散法 生物材料 

分 类 号：R318[医药卫生—生物医学工程]