中药复方癌宁对人胃腺癌细胞凋亡基因表达的影响  

Effect of Traditional Chinese Medicine Compounds Aining on the Expression of Apoptosis Inducing Genes of Human Gastric Cancer Cell

作　　者：苏勉诚 戴梅 吕晓英[3] 李红坤[4] 刘继林[4] 

机构地区：[1]广东省珠海市国家中药现代化工程技术研究中心,519020 [2]重庆市沙坪坝区中医院,400030 [3]四川大学公卫学院,成都610041 [4]成都中医药大学,610075

出　　处：《中药材》2002年第8期563-566,共4页Journal of Chinese Medicinal Materials

摘　　要：目的:探索癌宁对体外人胃癌细胞基因表达的影响。方法:采用流式细胞术,检测了中药复方癌宁及顺铂诱导胃癌细胞凋亡基因的表达。结果:顺铂、癌宁组与空白对照比较,bcl-2、c-myc基因表达产物含量显著降低,p^(53)、bax基因表达产物含量显著增高(P<0.01),bax/bcl-2比值显著增高。结论:癌宁对人胃癌细胞的诱导凋亡作用与其对癌基因、抑癌基因表达的改变有关。Objective: To investigate the effects of Aining on the genes of the human gastric cancer cells. Methods: Flow cytometry( FMC) was adopted to detect the gene expression of the gastric cancer cell in the Aining group, the cisplatin(DDP)group and the blank group. Results: Compared with the blank group, the expression of c-myc and bcl-2 genes was low, while that of the p53,bax genes was high(P< 0.01) ,and the ratio of bax/bcl-2 significantly increased in both the Aining group and the cisplatin(DDP)group. Conclusions: The mechanism of inducing the human gastric cancer cells apoptosis by Aining has some relationship with the changes of the genes.

关 键 词：胃腺癌 细胞凋亡 基因表达 中药复方 癌宁 药理学 流式细胞术 

分 类 号：R273.52[医药卫生—中西医结合] R285[医药卫生—中医肿瘤科]