葛根素和葛根总异黄酮的雌激素样活性  

Estrogen-like Effects of Puerann and Total Isoflavones from Pueraria lobata

作　　者：郑高利[1] 张信岳[1] 郑经伟[1] 孟倩超[1] 郑东升[2] 

机构地区：[1]浙江省医学科学院药物研究所,杭州310013 [2]浙江大学医学院附属第一医院,杭州310000

出　　处：《中药材》2002年第8期566-568,共3页Journal of Chinese Medicinal Materials

基　　金：浙江省自然科学基金(No:399037);浙江省医药卫生优秀青年科技人才专项科研基金

摘　　要：目的:研究葛根素和葛根总异黄酮的雌激素样活性。方法:去卵巢大鼠、幼年小鼠、成年小鼠和雌激素化小鼠灌胃给予葛根素和葛根总异黄酮150～600mg/kg,连续5～9天后,阴道脱落细胞检查和子宫重量法判断雌激素样作用。结果:葛根素和葛根总异黄酮能明显增加去卵巢大鼠阴道涂片中角化细胞数量,部分恢复去卵巢大鼠的性周期;使去卵巢大鼠和幼年小鼠子宫重量明显增加,这种作用呈明显的剂量依赖性。对正常成年小鼠的子宫生长无明显影响,在合用雌二醇时,葛根素和葛根总异黄酮均使雌二醇的促子宫生长作用明显减弱。结论:葛根素和葛根总异黄酮对雌激素低下动物显示弱雌激素活性,对正常雌激素水平动物无明显雌激素样活性,而在合用雌二醇时,则部分抑制雌二醇的促子宫生长作用,提示葛根素和葛根总异黄酮具有雌激素受体部分激动剂的特性。Objective:To study the estrogen-like effects of puerarin and total isoflavones from Pueraria lobata (TIP) in vivo. Methods: Puerarin and TIP were orally administrated to ovariectomized rats,infancy or adult mice and estrogen-treated mice at the doses of 150,300 and 600 mg/kg for 5-9 days.The estrogen-like effects were measured by viginacytology and uterus or ovary weights.Results:Puerarin and TIP significantly promoted uterus growth in ovariectomized rats and infancy mice, increased the ratios of keratocytes in vaginal smear in ovariectomized rats.The sexual cycle was partially recoveried in dose-dependent manner.In E2-treated mice,puerarin and TIP obviously inhibited the growth of vigina induced by E2. No obvious effect was observed in normal adult mice. Conclusion: The results showed that puerarin and TIP acted as weak estrogen-like effect on estrogen-dificiency animals,while no effect on normal-estrogen level ones,but as antiestrogen-like effect in high-estrogen-level ones.These results suggested that puerarin and TIP possessed property of partial agonist of estrogen receptor.

关 键 词：葛根总异黄酮 雌激素样活性 葛根素 药理学 雌激素受体激动剂 

分 类 号：R282.710.5[医药卫生—中药学]