富氢水对SD大鼠动脉化静脉桥再狭窄的作用  

Effect of hydrogen-rich water on arterialized vein graft restenosis in SD rats

作　　者：陈星权[1] 简杰[1] 郭惠明[1] 黄焕雷[1] 陈涌斌 卢聪[1] CHEN Xing-quan;JIAN Jie;GUO Hui-ming;HUANG Huan-lei;CHEN Yong-bin;LU Cong(Guangdong Cardiovascular Institute,Guangdong General Hospital,Guangdong Academy of Medical Sciences,Guangzhou 510100,China)

机构地区：[1]广东省心血管病研究所广东省人民医院(广东省医学科学院),广州510100

出　　处：《岭南心血管病杂志》2018年第3期333-338,共6页South China Journal of Cardiovascular Diseases

摘　　要：目的探究富氢水对SD大鼠动脉化静脉桥再狭窄的作用。方法 60只SD雌性鼠按随机数字表法分为3组,每组各20只,分别为空白对照组、脱氢水组、富氢水组,其中脱氢水组和富氢水组为实验组。对实验组建立SD大鼠股动脉静脉桥模型,模型建立后对富氢水组使用富氢水喂养,对空白对照组及脱氢水组则使用脱氢水喂养。实验6周后,标本取样并进行苏木素伊红染色观察内膜厚度、增殖细胞核抗原(proliferating cellnuclear antigen,PCNA)免疫组化观察、电镜下超微结构观察。结果术后6周,脱氢水组20例中5例、富氢水组20例中2例出现手术后右下肢肌肉萎缩,两组右下肢肌肉萎缩发生率比较差异无统计学意义(P=0.123)。空白组、富氢水组、脱氢水组内膜厚度分别为(34.5±11.1)μm、(42.3±8.0)μm、(56.9±12.5)μm,各组之间比较差异具有统计学意义(空白组vs.富氢水组,P=0.036;空白组vs.脱氢水组,P<0.001;富氢水组vs.脱氢水组,P=0.021)。空白组、富氢水组、脱氢水组3组PCNA高表达的比例逐步升高,且各组间比较均差异有统计学意义(空白组vs.富氢水组,P=0.013;空白组vs.脱氢水组,P<0.001;富氢水组vs.脱氢水组,P=0.023)。电镜下,与脱氢水组比较,富氢水组基底膜较完整,形成桥粒较少。结论富氢水能有效预防静脉移植物术后狭窄。Objectives To explore the effect of hydrogen-rich water on arterialized vein graft restenosis in SD rats.Methods We randomly divided 60 SD female rats into three groups（blank control group,dehydrogenation watergroup and hydrogen-rich water group）. Each group included 20 SD rats,among which dehydrogenation water groupand hydrogen-rich water group were the experimental group. Femoral artery vein bridge models of SD rats wereestablished for the experimental group. Hydrogen-rich water was used to feed the rats in hydrogen-rich water group.Dehydrogenation water was used to feed rats in blank control group and dehydrogenation water group. Six weeks afterthe experiment,endangium thickness of the sampled was observed by hematoxylin-eosin staining,expression of proliferatingcell nuclear antigen（PCNA）of the sampled was observed by immunohistochemical method,ultra microstructure of thesampled was observed by electron microscope. Results Six weeks after surgery,5 of the 20 cases in dehydrogenationwater group and 2 of 20 cases in hydrogen-rich water group were found to have a muscle atrophy in the right lowerextremity,with no statistical difference between the two groups（P = 0.123,chi-square test）. Endangium thicknesswere（34.5±11.1）μm in blank control group,（42.3±8.0）μm in hydrogen-rich water group,and（56.9±12.5）μm indehydrogenation water group,respectively,with significant differences among the three groups（blank control group vs.hydrogen-rich water group,P = 0.036;blank control group vs. dehydrogenation water group,P〈0.001;hydrogen-richwater group vs. dehydrogenation water group,P=0.021）. Proportion of high expression of PCNA in the three groupsincreased gradually,and there were statistical differences among the three groups（blank control group vs. hydrogenrichwater group,P=0.013;blank control group vs. dehydrogenation water group,P〈0.001;hydrogen-rich water groupvs. dehydrogenation group,P=0.023）. Under the electron microscope,basement membrane was more complete anddesmosome was le

关 键 词：富氢水 动脉化静脉桥 再狭窄 

分 类 号：R33[医药卫生—人体生理学]