N-乙酰半胱氨酸对哮喘小鼠气道上皮细胞自噬水平的影响  

Effect of N-acetylcysteine on Autophagy in Airway Epithelial Cells in Asthmatic Mice

作　　者：周冬梅 孔灵菲[1] ZHOU Dongmei,KONG Lingfei(Department of Respiratory and Critical Medicine, The First Hospital, China Medical University, Shenyang 110001, Chin)

机构地区：[1]中国医科大学附属第一医院呼吸与危重症医学科,沈阳110001

出　　处：《中国医科大学学报》2018年第7期626-630,共5页Journal of China Medical University

基　　金：沈阳市科学技术计划(F13-318-1-33)

摘　　要：目的探讨N-乙酰半胱氨酸(NAC)能否抑制支气管哮喘小鼠气道上皮细胞的自噬水平。方法将SPF级BALB/C小鼠随机分为正常对照组、支气管哮喘组及NAC治疗组。检测各组小鼠气道反应性及肺泡灌洗液细胞分类计数,肺组织切片行HE染色、Masson三色染色和AB-PAS染色评估气道炎症及气道重塑水平。LC3B免疫荧光染色、电镜及蛋白电泳观察气道上皮自噬体数量评估自噬水平。结果 NAC可显著抑制支气管哮喘的气道炎症,减轻气道上皮下的胶原沉积及黏液分泌。LC3B免疫荧光及蛋白电泳显示,NAC可降低气道上皮细胞的自噬水平,电镜结果显示NAC治疗组自噬体减少。结论 NAC可通过抑制气道上皮细胞的自噬水平减轻气道炎症及气道重塑。Objective To determine whether N-acetylcysteine（NAC） can inhibit autophagy in airway epithelial cells in bronchial asthma mice. Methods Specific pathogen free BALB/c mice were randomly divided into the health control group,the bronchial asthma group,and the NAC treatment group. Airway responsiveness and total cell count in bronchoalveolar lavage fluid were examined. Lung tissue sections were used for hematoxylin and eosin,Masson＇s trichrome,and Alcian blue/periodic acid-Schiff staining to evaluate airway inflammation and remodeling. Microtubule-associated proteins light chains 3B（LC3B） immunofluorescence staining,electron microscopy,and Western blotting were used to assess autophagy levels in airway epithelial cells. Results NAC significantly inhibited airway inflammation in bronchial asthma mice and reduced collagen deposition and mucus secretion in the airway epithelium. LC3B immunofluorescence and Western blotting analysis revealed that NAC reduced the level of autophagy in airway epithelial cells,and electron microscopy examination showed that the autophagosome number decreased in the NAC treatment group. Conclusion NAC can reduce airway inflammation and remodeling by inhibiting the autophagy in airway epithelial cells.

关 键 词：支气管哮喘 气道上皮细胞 自噬 N-乙酰半胱氨酸 

分 类 号：R562.25[医药卫生—呼吸系统]