有氧运动调控MAPK信号通路对于急性心肌缺血大鼠的作用  

Effects of Aerobic Exercise on Acute Myocardial Ischemia in Rats by Regulating MAPK Signaling Pathway

作　　者：龚彦豪 黄宝叶 杨新林 韦雪亮[3] GONG Yan-hao;HUANG Bao-ye;YANG Xin-lin;WEI Xue-liang(Department of Physical Education,Guilin University of Electronic Technology,Guilin,Guangxi,541004,China;Department of Physical Education,Northwest A＆F University,Yangling,Shaanxi,712100,China;Collegel of Physical Education,Guangxi University,Nanning,Guangxi,530004,China)

机构地区：[1]桂林电子科技大学体育部,广西桂林541004 [2]西北农林科技大学体育部,陕西杨凌712100 [3]广西大学体育学院,广西南宁530004

出　　处：《动物医学进展》2018年第6期71-74,共4页Progress In Veterinary Medicine

摘　　要：探讨有氧运动调控MAPK信号通路对于急性心肌缺血大鼠的作用。将30只健康雄性SD大鼠随机分成3组,对照组无运动处理。急性力竭组采用负重(力竭)游泳运动,有氧运动组采用无负重(有氧)游泳运动,均持续14d。采集试验前及试验后大鼠耳缘静脉血,检测血清肌酸激酶(CK)和乳酸脱氢酶(LDH)含量的变化,采用Western blot方法检测MAPK信号通路中ERK和p38MAPK蛋白表达。与对照组相比,急性力竭组大鼠CK和LDH血清含量显著升高;有氧运动组与急性力竭组相比,血清CK含量呈下降趋势;与对照组相比,急性力竭组大鼠心肌组织ERK和p38表达水平显著升高;有氧运动组与急性力竭组相比,心肌组织ERK和p38表达水平呈下降趋势(均P<0.05)。有氧运动对对急性力竭心脏损伤有保护作用,这一作用可能通过抑制MAPK信号通路中ERK和p38水平实现。To study the effects of aerobic exercise on acute myocardial ischemia in rats by regulating MAPK signaling pathway,thirty healthy male SD rats were randomly divided into three groups：control group（n=10）,acute exhaustive group（n=10）and aerobic exercise group（n=10）.The control group had no motor treatment.In the acute exhaustive group,weight-bearing（exhaustive）swimming was adopted,and swimming without load（aerobic）was used in the aerobic exercise group,both lasted for 14 days.The ear vein blood of rats was collected before and after the experiment.The changes of serum creatine kinase（CK）and lactate dehydrogenase（LDH）contents were measured in rats.The expressions of ERK and p38 MAPK proteins in MAPK signaling pathway were detected by Western blot.Compared with the control group,serum CK and LDH levels increased significantly in the acute exhaustive group;and compared with the acute exhaustive group,serum CK content decreased in the aerobic exercise group;compared with the control group,ERK and P38 in myocardial tissue of rats increased in the acute exhaustive group;expressions of ERK and P38 decreased in the aerobic exercise group than those in the acute exhaustive group.Aerobic exercise has a protective effect on acute exhaustive heart injury,which may be achieved by inhibiting the expression levels of ERK and p38 in the MAPK signaling pathway.

关 键 词：有氧运动 MAPK信号通路 急性心肌缺血 

分 类 号：R332[医药卫生—人体生理学]