利用SSR标记分析东北野生菰遗传多样性和遗传结构  

Genetic Diversity and Genetic Structure of Zizania latifolia Griseb(Turcz) from Northeast China Based on SSR Analysis

作　　者：苏晓娜[1] 王惠梅[2] 黄雪雯[3] 谢小燕 张孝天[4] 牛恋 董琳 江绍琳[5] 吴建利[2] 江绍玫[6] SU Xiao-na1 , WANG Hui-mei2, HUANG Xue-wen6, XIE Xiao-yan4, ZHANG Xiao-tian4, NIU Lian4, DONG Lin4, JIANG Shao-lin5, WU Jian-li2 ,JIANG Shao-mei3(1.Nanchang Business College of Jiangxi Agrieuhural University, Nanchang 330013, China ; 2. China National Rice Research Institute, Hangzhou 310006, China; 3.Qiannan Normal College for Nationalities, Duyun, Guizhou 558000, China; 4. College of Aart, Jiangxi University of Finance and Economics, Nanchang 330045, China ; 5. College of Agronomy, JAU, Nanchang 330045 ; 6. College of Statistics, Jiangxi University of Finance and Economics, Nanchang 330045, Chin)

机构地区：[1]江西农业大学南昌商学院,江西南昌330013 [2]中国水稻研究所,浙江杭州310006 [3]黔南民族师范学院,贵州都匀558000 [4]江西财经大学艺术学院,江西南昌330013 [5]江西农业大学农学院,江西南昌330045 [6]江西财经大学统计学院,江西南昌330013

出　　处：《江西农业大学学报》2018年第3期579-589,共11页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(31460378)~~

摘　　要：菰属(Zizania Trucz.)为水稻的近缘属,保留了大量驯化作物所丢失的优异性状,是扩大和丰富育种基因库的理想材料。从24对菰SSR分子标记中筛选出7对标记,对来自东北三省的5个居群,共计98个野生菰样本进行了遗传多样性和遗传结构分析。同时,详细阐述了POPGENE、STRUCTURE、ARLEQUIN、MEGA及MVSP等统计分析软件的数据录入格式、软件操作方法,为相关领域的研究者提供完整可操作的参考依据。各软件分析结果表明:东北地区野生菰具有较为丰富的遗传多样性(PPB=71.88%、Ao=1.719、Ae=1.332、He=0.201、I=0.313,Gst=0.543);各野生菰居群间的遗传相似性系数和遗传距离分别介于0.605 5~0.955 6和0.045 4~0.501 6;东北地区野生菰的遗传变异主要存在于居群间;5个野生菰居群的遗传结构可以大致分为3组,5个居群不存在明显的亲缘地理关系。该研究结果不仅有助于加深对我国东北地区野生菰资源遗传多样性和遗传结构的认识,同时也为东北野生菰种质资源保护提供一定理论依据。Zizania Latifolia（Griseb.） Trucz. is an aquatic herbaceous plant of Oryzeae tribe,which is a close genera of rice and an important carrier of genes controlling excellent agronomic traits of significant importance for rice breeding.In this study,seven out of 24 pairs of SSR markers were used for genetic diversity and genetic structure analysis of 5 wild populations from Northeast China. POPGENE,STRUCTURE,ARLEQUIN,MEGA and MVSP,were introduced for its data format and usage methods.This work showed that Z.latifolia resources in Northeast China had a rich genetic diversity. The results of POPGENE analysis showed that there was rich genetic diversity in the wild species（PPB = 71.88 %、Ao = 1.719、Ae = 1.332、He = 0.201、I = 0.313,Gst = 0.543） and the genetic similarity coefficient and genetic distance were between 0.6055 - 0.9556 and 0.0454 - 0.5016,respectively.Based on molecular variation analysis by ARLEQUIN,it was showed that the genetic variation of the 5 wild populations mainly exists among populations while low degree of variation was tested within populations.For the genetic structure analysis,5 populations were divided into three groups.Cluster analysis showed that the 5 wild populations of Z.latifolia could be classified into two groups（one group was DL,and the other was SY,QQHE,JMS,TH）. There was no significant association between the phylogenetic relationship and the geographic locations in the Z.latifolia populations.This study deepens our understanding about the genetic evolution of Z.latifolia in Northeast China and at the same time provides certain theoretical basis for protection of this wild rice germplasm resource.

关 键 词：野生菰 SSR标记 生物统计分析软件 遗传多样性 遗传结构 

分 类 号：Q943[生物学—植物学]