抽真空法和甘油冷冻法保存对高效脱胶菌株CXJZU-120活化性能的比较研究  

Performance Comparison of the Strain CXJZU-120 Preserved by the Methods of Vacuum Pumping and Glycerol Cryopreservation

作　　者：冯湘沅[1] 成莉凤[1] 刘正初[1] 郑科[1] 杨琦[1] 刘志远[1] 曾洁[1] 段盛文[1] 彭源德[1] FENG Xiangyuan;CHENG Lifeng;LIU Zhengchu;ZHENG Ke;YANG Qi;LIU Zhiyuan;ZENG Jie;DUAN Shengwen;PENG Yuande(Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha 410205,China)

机构地区：[1]中国农业科学院麻类研究所,长沙410205

出　　处：《中国麻业科学》2018年第3期106-110,共5页Plant Fiber Sciences in China

基　　金：中国农业科学院科技创新工程(ASTIP-IBFC08);现代农业产业技术体系建设专项(No.CARS-16-E22);国家自然科学基金(31700438);湖南省自然科学基金(No.2016jj3126)

摘　　要：为了探索一种使高效脱胶菌株能够长期保存且易活化的方法,试验采用抽真空法和甘油冷冻法两种方法对保存了3年或10年的菌株CXJZU-120进行苎麻脱胶,定期测定活化菌液和发酵液中相关活化性能指标。结果表明:甘油冷冻法保存3年的菌株经活化10 h,菌体颜色深浅不一;发酵10.5 h,其活菌数为1.66×10~7cfu/mL,COD值为3.86 g/L,苎麻脱胶失重率为19.53%,只适合短期保存。然而,抽真空法保存3年、10年的两支菌株经活化10 h,活化菌体颜色均较深;发酵10.5 h,其活菌数均可达1.02×10~9cfu/mL,COD值分别为6.02、5.65 g/L,苎麻脱胶失重率均超过27%。因此,抽真空法适合高效脱胶菌株CXJZU-120中、长期保存。In order to explore a long-term preservation and easily activating method for high efficiency degumming strain,the related activation performance indexes of activated bacteria and fermentation liquid were tested during ramie degumming using the strain CXJZU-120 preserved for 3 years or10 years by the method of vacuum and glycerol cryopreservation. After the 10 h activation of the strain preserved for 3 years by the method of glycerol cryopreservation,the results showed that the strain color was different,the number of viable bacteria was 1. 66 × 10~7 cfu/mL,COD value was 3. 86 g/L,and the loss rate of ramie degels was 19. 53%,which was indicated that the method was only suitable for short-term preservation. However,after the activation of the strain preserved for 3 years and 10 years by the vacuum method,the strain color was deep,the number of viable bacteria was 1. 02 × 10~9 cfu/mL,COD value were 6. 02 g/L and 5. 65 g/L respectively,and the loss rate of ramie degel was over 27%. Therefore,the vacuum method was suitable for the long-term preservation of the strain CXJZU-120.

关 键 词：高效脱胶菌株CXJZU-120 保存方法 活化性能 

分 类 号：Q939.9[生物学—微生物学]