PEG模拟水分胁迫对白菜型冬油菜幼苗生长及生长特性的影响  

Response of winter rapessed seeding growth and physiological characteristics under PEG drought tolerance

作　　者：董小云 米超[1,2] 刘自刚 孙万仓[1,2] 武军艳 方彦[1,2] 李学才 赵艳宁[1,2] 刘海卿 DONG Xiaoyun;MI Chao;LIU Zigang;SUN Wancang;WU Junyan;FANG Yan;LI Xuecai;ZHAO Yanning;LIU Haiqing(College of Agronomy,Gansu Agricultural University,Gansu Province Key Laboratory of Arid Land Crop Sciences,Improvement and Key Laboratory of Crop Genetics and Germplasm Enhancement,Rapeseed Engineering Research Center of Gansu Province,Lanzhou 730070,Chin)

机构地区：[1]甘肃农业大学农学院,甘肃省干旱生境作物学重点实验室 [2]甘肃省作物遗传改良与种质创新重点实验室,甘肃省油菜科技研究工程中心,甘肃兰州730070

出　　处：《河南农业大学学报》2018年第3期313-321,共9页Journal of Henan Agricultural University

基　　金：国家现代农业产业技术体系(CARS-12); 国家自然科学基金项目(31460356); 国家“973”计划(2015CB150206); 国家863计划(2011AA10A104); 国家农业科技成果转化项目(2014G10000317); 丝绸之路经济带新疆核心区农田周年覆盖高效种植模式研究与示范项目(2016E02009)

摘　　要：采用不同质量分数的聚乙二醇6000(Polyethylene glycol 6000,PEG6000)溶液模拟水分胁迫,研究4种白菜型冬油菜幼苗形态特征及生理生化特性,评判不同品种的抗旱性强弱。结果表明,PEG对4种白菜型冬油菜的成苗率、主根长、苗高、单株鲜质量、相对活力指数有显著的抑制效应;PEG质量分数为5%~15%时,超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性与胁迫质量分数呈正相关关系,在质量分数15%~20%时酶活性出现峰值,达显著性水平(P<0.05),随之下降。可溶性糖(SS)含量、可溶性蛋白(SPr)含量、脯氨酸(Proline)含量、相对电导率、丙二醛(MDA)含量与胁迫质量分数呈显著正相关关系。线性回归分析得到白菜型冬油菜PEG胁迫的半致死质量分数(LC50)为18.2%、半致死相对电导率(EC of LC50)为15.73%。4个品种的抗旱性顺序依次为:陇油6号>陇油7号>天油7号≥天油5号。Different mass fractions of polyethylene glycol 6000（ PEG6000） solution were used to simulate the water stress. The morphological characteristics and physiological and biochemical characteristics of four B. campestris were studied and the drought resistance of different varieties was evaluated. The results showed that PEG had a significant inhibitory effect on seedling rate,main root length,seedling height,fresh weight per plant,and relative vigor index of four kinds of winter campestris. At 5% to 15% PEG mass fraction,there was a positive correlation between superoxide dismutase（ SOD） activity,peroxidase（ POD） activity,catalase（ CAT） activity and stress massfraction,while at a mass fraction of 15% to 20%,the enzyme activity peaked,reaching a significant level（ P≤0. 05）,followed by a decrease. There was a significant positive correlation between soluble sugar（ SS） content, soluble protein（ SPr） content, proline content, relative conductivity,malondialdehyde（ MDA） content and stress mass fraction. Linear regression analysis showed that the semi-lethal mass fraction（ LC50） of PEG stress was 18. 2% and the EC of LC50 was 15. 73%. The order of drought resistance of the four varieties was as follows： Longyou 6 Longyou 7 Tianyou 7≥Tianyou 5.

关 键 词：水分胁迫 白菜型冬油菜 形态特征 生理生化特征 半致死浓度 

分 类 号：S634[农业科学—蔬菜学]