具有梯度孔结构特征的聚己内酯多孔支架的3D打印制备及表征  

Fabrication and Characterization of 3D Printed Poly(ε-Caprolactone) Porous Scaffolds with Designed Gradient Pore Sizes

作　　者：张豪杰 邢天龙 周燕[1] 谭文松[1] 叶朝阳[1] ZHANG Hao-jie;XING Tian-long;ZHOU Yan;TAN Wen-song;YE Zhao-yang(State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,Chin)

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《高校化学工程学报》2018年第3期708-717,共10页Journal of Chemical Engineering of Chinese Universities

基　　金：国家自然科学基金(81671841);上海市自然科学基金(16ZR1408700)

摘　　要：软骨组织工程生物支架材料的内部结构(如孔结构)对于细胞行为有着重要影响。3D打印技术可以实现对支架结构的精确控制。聚己内酯(poly(ε-caprolactone),PCL)是广泛应用的组织工程支架材料,具有良好的生物相容性,支持多种细胞的黏附、增殖和基质分泌。3D打印制备的PCL多孔支架包含相邻三部分,每部分由六层纤维堆积,相邻两层的纤维夹角分别为30、45和60°,而同层中纤维间距为分别为450、400和300μm;力学性能测试梯度支架的杨氏模量在47 MPa左右,与天然软骨组织20 MPa相匹配;微断层扫描和扫描电镜观察支架表面和内部形貌的结构特征,Image J软件对支架特征参数进行量化分析。支架表面和内部形貌规整,呈现孔结构梯度分布,具有良好的孔连通性和57%左右的孔隙率,孔径梯度大小为342~747μm。经细胞接种培养后,应用扫描电镜、CCK8检测和死活染色方法分析支架材料对细胞生长及活性的影响。梯度支架能够很好地支持细胞生长和活性。研究通过对支架材料的设计和制备为软骨组织工程研究奠定了基础。The microstructure of biomaterial scaffolds such as pore structure plays an important role in cellular behaviors in cartilage tissue engineering. 3D printing can be applied to fabricate tissue engineering scaffolds with well-designed structures. Poly（ε-caprolactone）（PCL） is widely used in tissue engineering due to its biocompatibility and capability of supporting cell adhesion, proliferation and extracellular matrix production.PCL porous scaffolds were designed and fabricated by 3D printing. The scaffolds had three integrated parts with alignment angles between fibers in adjacent layers as 30, 45 and 60°, and the distances between neighboring fibers in the same layer are 450, 400 and 300 μm, respectively. The Young＇s modulus of gradient scaffolds was determined by mechanical properties at 47 MPa, which was similar to 20 MPa of native cartilage tissue. μ-CT and scanning electron microscopy were used to characterize the surface and internal structures, and the structural parameters were quantitatively analyzed by Image J software. The surface and internal morphologies of the scaffolds are intact with gradient pore structure, good pore interconnectivity and 57% porosity. The pore size is between 342~747 μm. Cell growth and viability were analyzed after cell culture by scanning electron microscopy, CCK8 assay and live dead staining. The gradient scaffold could support cell proliferation and viability.The present study is helpful for fabricating cartilage tissue engineering scaffolds with well controlled microstructural features.

关 键 词：3D打印 聚己内酯 孔结构 梯度支架 软骨组织工程 

分 类 号：R318.08[医药卫生—生物医学工程] Q28[医药卫生—基础医学]