中国美味蘑菇野生菌株的鉴定、生物学特性及驯化研究  

Identification,Biological Characteristics and Artificial Cultivation of Wild Agaricus sinodeliciosus

作　　者：徐鸿雁 刘海林 罗春燕 XU Hongyan;LIU Hailing;LUO Chunyan(Edible Fungus Research,Institute of Wild Plant Resources in Oing-Tibet Plateau,Academy of Agriculture and Forest,Oinghai University,Xining,Oinghai 810016,China)

机构地区：[1]青海大学农林科学院青藏高原野生植物资源研究所,青海西宁810016

出　　处：《食用菌学报》2018年第2期56-61,共6页Acta Edulis Fungi

基　　金：青海省农业科技成果转化资金项目(2014GB2G200065)

摘　　要：对从青海省海西州诺木洪林场草滩地采集获得的野生子实体进行鉴定,该野生子实体为中国美味蘑菇(Agaricus sinodeliciosus),并对其分离纯化菌株进行生物学特性和驯化研究。研究结果表明,该菌株菌丝在PDA培养基(200g马铃薯,17g葡萄糖,17g琼脂粉,1000mL水)上长势较好,生长快,日生长速度为(1.755±0.1)mm/d;菌丝生长的最适温度为25℃,培养基初始pH为8.0;使用棉籽壳培养料(61%棉籽壳,26%发酵牛粪,9.8%麸皮,0.8%石灰,0.8%石膏,0.8%过磷酸钙,0.8%轻质碳酸钙)栽培,覆土17d后即可出菇,子实体为白色伞状,单菇重(72.00±1.22)g。A wild mushroom was collected from the wild grasslands of Nomuhong Forestry Centre in Haixi, Qinghai Province, purified to obtain strain CDM-1 and then identified to be Agaricus sinodeliciosus. The mycelium of CDM-1 grew well on PDA medium （200 g potato, 17 g glucose, 17 g agar powder, and 1000 mL water）, and the mycelial growth speed was 1. 755 ＋ 0.1 mm/d. The optimal temperature and pH for the mycelial growth of CDM-1 were 25~C and 8. 0, respectively. For the artificial cultivation of CDM-1, a cottonseed hull substrate containing 61% cottonseed hull, 26% fermented cow manure, 9.8% bran, 0.8% lime, 0.8% gypsum, 0.8% calcium superphosphate, and 0.8% light calcium carbonate was used. Fruiting bodies of cultivated CDM-1 were obtained after covering casing soil for 17 days, and the cultivated fruiting bodies were white and umbrella-shaped with an average weight of （72. 00 ＋ 1. 22） g for individual mushrooms.

关 键 词：形态特征 序列比对 菌丝生物学特性 

分 类 号：S646[农业科学—蔬菜学]