HPLC法同时测定鼻炎灵片中绿原酸、黄芩苷和欧前胡素的含量  

Simultaneous Determination of Chlorogenic Acid,Baicalin and Imperatorin in Biyanling Tablets by HPLC

作　　者：邱红梅[1] 杨林[2] 何丹[1] QIU Hongmei1,YANG Lin2,HE Dan1(1. College of Pharmacy,Chongqing Medical University,Chongqing400016,China;2. Dept. of Pharmacy,Chongqing Medical and Pharmaceutical College,Chongqing 401331,Chin)

机构地区：[1]重庆医科大学药学院,重庆400016 [2]重庆医药高等专科学校药学系,重庆401331

出　　处：《中国药房》2018年第12期1605-1608,共4页China Pharmacy

基　　金：国家自然科学基金资助项目(No.31400881);重庆市基础科学与前沿技术研究项目(No.cstc2017jcyj AX0211)

摘　　要：目的:建立同时测定鼻炎灵片中绿原酸、黄芩苷和欧前胡素含量的方法。方法:采用高效液相色谱法。色谱柱为Waters Sun Fire C18,流动相为甲醇-0.20%磷酸溶液(梯度洗脱),流速为1.0 mL/min,检测波长为280 nm,柱温为30℃,进样量为20μL。结果:绿原酸、黄芩苷和欧前胡素的检测质量浓度线性范围分别为1.618~16.18、1.624~16.24、1.762~17.62μg/mL(r均为0.9999);定量限分别为2.67、2.03、1.87μg/mL,检测限分别为1.34、1.06、0.98μg/mL;精密度、稳定性、重复性试验的RSD均小于2.0%;加样回收率分别为98.01%~101.80%(RSD=1.38%,n=9)、98.38%~101.40%(RSD=1.26%,n=9)、98.06%~101.40%(RSD=1.28%,n=9);耐用性试验的RSD均小于2.0%。结论:该方法操作简便、准确,精密度、稳定性、重复性、耐用性好,可用于鼻炎灵片中绿原酸、黄芩苷和欧前胡素含量的同时测定。OBJECTIVE： To establish a method for simultaneous determination of chlorogenic acid, baicalin and imperatorin in Biyanling tablets. METHODS：HPLC method was adopted. The separation was performed on a Waters Sun Fire C18 column with mobile phase consisted of methanol-0.20% phosphoric acid（gradient elution）at the flow rate of 1.0 mL/min.The detection wavelength was set at 280 nm and column temperature was 30 ℃. The sample size was 20 μL. RESULTS：Linear ranges of chlorogenic acid,baicalin and imperatorin were 1.618-16.18 μg/mL,1.624-16.24 μg/mL and 1.762-17.62 μg/mL（r were 0.999 9）,respectively. The limits of quantitation were 2.67,2.03,1.87 μg/mL,and the limits of detection were 1.34,1.06,0.98 μg/mL,respectively. RSDs of precision,stability and reproducibility tests were all lower than 2.0%. The recoveries were 98.01%-101.80%（RSD=1.38%,n=9）,98.38%-101.40%（RSD=1.26%,n=9）and 98.06%-101.40%（RSD=1.28%,n=9）,respectively. RSDs of tolerance tests were all lower than 2.0%. CONCLUSIONS：The method is simple,accurate,precise,stable,reproducible and durable. It can be used for simultaneous determination of hlorogenic acid,baicalin and imperatorin in Biyanling tablets.

关 键 词：高效液相色谱法 鼻炎灵片 绿原酸 黄芩苷 欧前胡素 含量测定 

分 类 号：R927.2[医药卫生—药学]