感染鸭源小鹅瘟病毒QH-L01株樱桃谷雏鸭病理学研究  

Pathological study on duck-origin goose Parvovirus strain QH-L01 infection of Cherry Valley duck

作　　者：陈兵[1] 徐婧[1,2] 罗启慧[1,2] 黄超[1] 刘文涛[1,2] 吴洪[2] 陈镜妃 陈苹[1] 高琪[1] 马文静[1] 陈正礼[1,2] CHEN Bing;XU Jing;LUO Qihui;HUANG Chao;LIU Wentao;WU Hong;CHEN Jingfei;CHEN Ping;GAO Qi;MA Wenjing;CHEN Zhengli(Laboratory of Animal Disease Model,College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,China;Key Laboratory of Animal Disease and Human Health of Sichuan Province,Sichuan Agricultural University,Chengdu 611130,China)

机构地区：[1]四川农业大学动物医学院,实验动物疾病模型实验室,四川成都611130 [2]四川农业大学动物疫病与人类健康四川省重点实验室,四川成都611130

出　　处：《浙江农业学报》2018年第6期932-938,共7页Acta Agriculturae Zhejiangensis

基　　金：国家科技支撑计划(2014BAI03B01); 国家重大科学仪器设备开发专项(2013YQ49085906)

摘　　要：探究人工感染条件下,鸭源小鹅瘟病毒QH-L01株对樱桃谷雏鸭各器官组织病理学变化的影响,以期阐明QH-L01的病理发生规律及特征。实验组以每只0.2 m L(10^(6.54)ELD_(50))的剂量肌肉接种30只2日龄樱桃谷肉鸭鸭源小鹅瘟病毒,对照组的鸭接种等量的灭菌生理盐水,分别在1、3、6、9 d观察各组织器官的病理变化。感染后1 d,先出现肺点状出血,部分肺小叶扩张,十二指肠、回肠和盲肠等肠道组织绒毛上皮细胞坏死脱落。感染后3 d,脑、肾、胸腺、法氏囊出现病理变化,十二指肠、回肠和盲肠等肠道组织绒毛和黏膜层轻度坏死、脱落。感染后6 d,脾脏淋巴细胞增多,十二指肠、回肠和盲肠等肠道组织绒毛和黏膜层中度坏死、脱落。感染后9 d,各组织病变减轻或者恢复正常。整个感染期死亡率为20%。In the present study,the histopathological changes of various organs of Cherry Valley ducklings were explored under the condition of artificial infection,to clarify the pathogenesis and characteristics of duck-origin goose Parvovirus strain QH-L01.Thirty Cherry Valley ducks in experimental group were intramuscularly inoculated with0.2 m L of 10^（6.54）ELD_（50）duck-origin goose Parvovirus strain,and the ducks in the control group were intramuscularly inoculated with 0.2 m L of sterilized normal saline per duck,to observe the pathological changes of various tissues/organs in each infective stage at 1,3,6,9 d,respectively.On the 1^（st）day after infection,the lungs appeared punctate hemorrhage,partial lobular expansion,and intestinal tissue villous epithelial cells necrosed.On the 3^（rd）day after infection,brain,kidney,thymus,bursa fabricius showed pathological changes,and villus of duodenum and ileum slight necrosed,mucosa layer of cecum appeared slight necrosis.On the 6^（th）day after infection,lymphocytes in spleen increased,moderate necrosis was detected in villus of duodenum,ileum and mucosa layer of cecum.On the9^（th）day after infection,the lesions were reduced or returned to normal.The mortality rate was 20%during the whole infection period.

关 键 词：鸭源小鹅瘟病毒 樱桃谷肉鸭 病理变化 损伤 

分 类 号：S855.3[农业科学—临床兽医学]