鲜切竹笋伤害信号的初步研究  

Preliminary research on wound signal in fresh-cut bamboo shoots

作　　者：何雄[1] 肖尚月 金图南 罗海波 孙金才[1] HE Xiong;XIAO Shangyue;JIN Tunan;LUO Haibo;SUN Jincai(Faculty of Food Scienee,Zhejiang Pharmaceutical College,Ningbo 315100,China;Ginling College,Nan-ring Normal University,Nanjing 210097,China)

机构地区：[1]浙江医药高等专科学校食品学院,浙江宁波315100 [2]南京师范大学金陵女子学院,江苏南京210097

出　　处：《浙江农业学报》2018年第6期992-998,共7页Acta Agriculturae Zhejiangensis

基　　金：浙江省教育厅科研项目(Y201432415);浙江省重点研发项目(2017C02021)

摘　　要：初步研究鲜切处理对距竹笋切割位点不同距离切段中伤害信号传递的影响,并通过外源信号分子茉莉酸甲酯(methyl jasmonate,Me JA)及其合成抑制剂水杨苷异羟肟酸(salicylhydroxamic acid,SHAM)处理,探索Me JA在鲜切竹笋信号分子合成和传递中的作用。结果表明:在切割位点产生了伤害信号物质,并能传导至邻近未受伤组织中,诱导苯丙氨酸解氨酶(phenylalanine ammonia lyase,PAL)、过氧化物酶(peroxidase,POD)活性的升高。Me JA处理能引起鲜切竹笋PAL、POD、超氧化物歧化酶(superoxide dismutase,T-SOD)和抗坏血酸过氧化物酶(ascorbate peroxidase,APX)活性升高,而SHAM处理对上述酶的活性升高具有一定的抑制作用,表明Me JA可能诱导并参与了鲜切竹笋在受到机械伤害后某种伤害信号的合成和传导。In order to explore the wound signal induced by mechanical damage in fresh-cut bamboo shoots,the effect of fresh-cut treatment on the damage signal transmission of bamboo shoots at different distances from the cutting site and the influence of methyl jasmonate（ Me JA） on the synthesis Me JA and transmission of signal molecule in freshcut bamboo shoots were investigated with the exogenous signal molecule and its synthetic inhibitor salicylhydroxamic acid（ SHAM）. The results showed that the wound signal substance was generated at the cleavage site,and it could be transmitted to the adjacent uninjured tissue to increase the activities of phenylalanine ammonia lyase（ PAL） and peroxidase（ POD）. Me JA treatment could increase the activities of PAL,POD,superoxide dismutase（ T-SOD） and ascorbate peroxidase（ APX） in fresh-cut bamboo shoots,while SHAM treatment had a certain inhibitory effect.Thus,it was hypothesized that Me JA could induce and participate in the synthesis and transmission of some damage signal after mechanical damage in fresh-cut bamboo shoots.

关 键 词：植物生理 茉莉酸甲酯 竹笋 信号传导 

分 类 号：Q945[生物学—植物学] S644.2[农业科学—蔬菜学]