白头翁皂苷D体外抗肺腺癌作用及其机制  

Antitumor effect and mechanism of pulchinenoside D on A549 tumor cells in vitro

作　　者：王彦儿 岳文华[2] 许琼明[2] 李笑然[2] 李坤平 WANG Yaner;YUE Wenhua;XU Qiongming;LI Xiaoran;LI Kunping(School of Pharmacy Guangdong Pharmaceutical University Guangzhou 510006 China;College ofPharmaceutical Science Soochow University Suzhou 215123 China)

机构地区：[1]广东药科大学药学院,广东广州510006 [2]苏州大学药学院,江苏苏州215123

出　　处：《广东药科大学学报》2018年第3期316-319,共4页Journal of Guangdong Pharmaceutical University

基　　金：国家自然科学基金项目(81273402;81573548)

摘　　要：目的探讨白头翁皂苷D体外抗肺腺癌细胞A549的作用及机制。方法采用MTT法、集落形成实验对白头翁皂D体外抗A549细胞活性进行研究;采用流式细胞仪检测细胞凋亡率;使用蛋白印迹法检测A549细胞中线粒体凋亡途径及PI3K-Akt-m TOR信号通路相关蛋白的表达。结果白头翁皂苷D(20、40μmol/L)体外可诱导肺腺癌细胞A549凋亡;蛋白印迹实验结果显示白头翁皂苷D(20、40μmol/L)可下调线粒体凋亡途径相关蛋白bcl-2、caspase-3和PI3K/Akt/mTOR信号传导通路主要相关蛋白PI3K、p-Akt、p-m TOR和p-p70S6K的表达。结论白头翁皂苷D有显著的体外抗肺腺肿瘤作用,其作用机制与下调线粒体凋亡途径和PI3K/Akt/m TOR信号通路相关蛋白的表达有关。Objective To study the antitumor effect and mechanism of pulchinenoside D on human lung tumor A549 cells in vitro. Methods MTT assay was used to evaluate the increment restrain of pulchinenoside D on A549 cells. Colony formation units were used to examine colony-forming role by pulchinenoside D on A549 cells. Flow cytometry assay was used to analyze the apoptosis rate of A549 cells.Western blotting was employed to determine the expression levels of bcl-2, caspase-3 and the proteins related to the PI3 K/Akt/m TOR signal in A549 cells. Results Pulchinenoside D restrained the proliferation of A549 cells,and significantly inhibited the colony formation of A549 cells. Pulchinenoside D at 20 and 40μmol/L significantly induced the apoptosis of A549 cells. Pulchinenoside D at 20 and 40 μmol/L reduced the expression of bcl-2 and caspase 3. Conclusion Pulchinenoside D displays antitumor effects on A549 tumor cells in vitro. The mechanism of which is related to regulation of the mitochondrial apoptosis pathway and PI3 K/Akt/m TOR signal.

关 键 词：白头翁皂苷D 肺腺癌 凋亡 细胞周期 PI3K-Akt-mTOR途径 

分 类 号：R285.5[医药卫生—中药学]