蓝花丹叶片不定根的诱导与光质对其白花丹素积累影响  

Adventitious Roots Induction from Leaves Explants in vitro and Effect of Light Quality on Its Plumbagin Accumulation in Plumbago auriculata Lam.

作　　者：朱原 高素萍[1,2] 罗良旭 胡菊[1] 雷霆[2] 付晓凤 ZHU Yuan;GAOSu-ping;LUO Liang-xu;HU Ju;LEI Ting;FU Xiao-feng(College of Landscape Architecture,Sichuan Agricultural University,Chengdu 611130,China;Garden Research Institute,Sichuan Agricultural University,Chengdu 611130,China;Forestry College,Guangxi University,Nanning 530004,China)

机构地区：[1]四川农业大学风景园林学院,成都611130 [2]四川农业大学园林研究所,成都611130 [3]广西大学林学院,南宁530004

出　　处：《四川农业大学学报》2018年第3期337-343,共7页Journal of Sichuan Agricultural University

基　　金：四川省应用基础研究项目(17YYJC0121);四川省科技计划项目(2017NZ0008)

摘　　要：【目的】探索一种由叶片直接诱导不定根的方法,并试图回答光质是否能促进蓝花丹不定根中白花丹素积累。【方法】首先以无菌叶片为组培外植体,采用NAA和KT激素组合直接诱导出不定根,在此基础上,用不同的光质(红光、蓝光、不同比例的红蓝复合光)处理不定根,测定其干物质及白花丹素积累量。【结果】结果表明:(1)所有添加NAA处理均能直接诱导叶片产生不定根,未添加NAA处理生根率为零。NAA中添加KT会延迟不定根发生的时点,但能产生更多的不定根;(2)单蓝光抑制蓝花丹叶片不定根干物质的积累,单红光则没有显著影响,复合作用后,红蓝光3∶1与1∶1时呈现出促进作用,随着蓝光比例的增加又显现为抑制作用;(3)单蓝光、红蓝光1∶3、1∶1的促进不定根中白花丹素的积累作用最强,红光比例超过蓝光时则不利白花丹素的积累,单红光也有同样的不利作用。所有处理的白花丹素含量均呈现先增后减的趋势,第30天时的含量最高。【结论】在蓝花丹叶片诱导不定根发生过程中NAA是主导激素,KT与NAA交互作用能一定程度上提高不定根产量,故以NAA 0.5 mg/L+KT 0.1 mg/L诱导蓝花丹叶片不定根发生的效果最佳。光质对蓝花丹不定根中次生代谢产物积累以及生长发育有着显著影响,且红、蓝光对它们的作用呈现相反的作用。红、蓝光1∶1的光质对不定根连续处理30 d可得到单位时间含量较高的白花丹素。【Objective】The aim of this study was to explore a method of direct induction of adventitious roots from leaves and to answer whether the light quality could promote the accumulation of plumbagin in adventitious roots of P. auriculata.【Method】The adventitious roots were induced by NAA or/and KT from leaves explants in vitro and they were cultivated under with different light quality（red or/and blue）.Dry mass and plumbagin were measured. 【Results】（1） Leaves explants could directly induce adventitious roots among all treatments supplied with NAA but the rooting rate of explants was zero without NAA.（2） Single blue light inhibited the accumulation of dry matter of adventitious roots. However,no significant effect was found in single red light treatment. Both red-blue light with ratio of 3∶1 and 1∶1 showed promoting effect. The inhibition effect seemed to increase with increasing blue light ratio.（3） Single blue light,red-blue light with ratio of 1 ∶3 and 1 ∶1 had stronger promotion effect than other light quality in the accumulation of plumbagin. However,there were negative effects when red light ratio exceeded blue light ratio. Single red light also had similar adverse effects. The content of plumbagin tended to increase first and then decrease in all treatments,with maximum value appearing on 30 days.【Conclusion】In addition,NAA was the dominant hormone during the process of adventitious root induction. The interaction between KT and NAA could,to some extent,increase the yield of adventitious root. The combine of 0.5 mg/L NAA and 0.1 mg/L KT achieve was the best to induce adventitious roots from leaves. The light quality had a significant effect on the accumulation of secondary metabolites and the growth and development in adventitious roots. However,red light had an opposite effect compared with blue light. Red-blue light with 1∶1 ratio on 30 days obtained highest content of plumbagin in adventitious roots.

关 键 词：蓝花丹 白花丹素 不定根 光质 外源激素 

分 类 号：Q945.79[生物学—植物学]