唐家河国家级自然保护区中华蜜蜂(Apis cerana cerana)的线粒体遗传多样性分析  

Genetic Diversity of Apis cerana cerana Based on Mitochondrial DNA in Tangjiahe National Nature Reserve,Sichuan,China

作　　者：李彪[1] 赖康[2] 鲜方海 向国栋 周明 欧阳菠 钟赟 余姣姣 杨建东[1] LI Bioo;LAI Kong;XIAN Fang-hoi;XIANG Guo-dong;ZHOU Ming;OUYANG Bo;ZHONG Yun;YU Jiao-jiao;YANG Jian-dong(College ofAnimal Science and Technology,Sichuan Agriculture University,Chengdu 611130,China;Sichuan Province Apiculture Management Station,Chengdu 610042,China;Tangjiahe Resources Development Limited Liability Company,Guangyuan 628109,Sichuan,China;Pingwu County Forestry Bureau,Mianyang 622550,Sichuan,China)

机构地区：[1]四川农业大学动物科技学院,成都611130 [2]四川省蜂业管理站,成都610042 [3]青川县唐家河野生资源开发有限责任公司,四川广元628109 [4]平武县林业局,四川绵阳622550

出　　处：《四川农业大学学报》2018年第3期386-391,共6页Journal of Sichuan Agricultural University

基　　金：四川省"十三五"农畜育种攻关项目(2016NYZ0048);四川省教育厅创新团队项目(14TD0002)

摘　　要：【目的】了解唐家河国家级自然保护区中华蜜蜂的遗传多样性,为进一步保护和利用唐家河中华蜜蜂资源提供科学依据。【方法】以基因片段mt DNA t RNAleu^COⅡ的非编码区作为分子标记,对唐家河各个地区共37个中蜂样本进行序列测定及相关分析。【结果】唐家河中蜂的核苷酸多样度(Pi)为0.030 34,单倍型多样性(Hd)为0.839±0.041;共发现14个单倍型,主体单倍型TJH2和TJH4分别占总样本数的29.73%和27.03%。此外本研究首次报道了3个新发现的单倍型。【结论】唐家河中蜂具有丰富的遗传多样性,其单倍型的分布与地理、气候等因素有较大关系。【Objective】The purpose of this study was to understand the genetic diversity and provides the scientific basis for protecting and utilizing the resources of Apis cerana cerana in Tangjiahe National Nature Reserve,Sichuan,China.【Method】The mitochondrial DNA t RNAleu～CO Ⅱ sequences of 37 samples from Tangjiahe National Nature Reserve were determined and analyzed.【Result】It was shown that the nucleotide polymorphism（Pi） was 0.030 34 and the haplotype diversity（Hd） was 0.839±0.041;14 haplotypes were found in 37 honeybee colonies. TJH2（29.73%） and TJH4（27.03%） were the main haplotypes found in the 37 honeybee colonies. Moreover,three new haplotypes were found in Tangjiahe National Nature Reserve. 【Conclusion】Tangjiahe National Nature Reserve has abundant genetic diversity and the haplotype distribution,which was connected with geography,climate and other factors. It provides important theoretical basis for the protection and reasonable using of the genetic resources of Apis cerana cerana in Tangjiahe National Nature Reserve.

关 键 词：中华蜜蜂 唐家河国家级自然保护区 遗传多样性 

分 类 号：Q963[生物学—昆虫学]