XN-20 WPC对外周血原始细胞与异常淋巴细胞检测的价值  

XN-20 WPC for the determinations of blast cells and abnormal lymphocytes in peripheral blood

作　　者：夏永辉 韩庆庆 王守磊 李勇[1] XIA Yonghui;HAN Qingqing;WANG Shoulei;LI Yong(Department of Clinical Laboratory,Institute of Hematology,Chinaese Academy of Medical Sciences,Tianjin 300020,China)

机构地区：[1]中国医学科学院血液病医院检测中心,天津300020

出　　处：《检验医学》2018年第6期469-471,共3页Laboratory Medicine

摘　　要：目的采用XN-20血液分析仪(简称XN-20)白细胞前体通道(WPC)检测外周血原始细胞及异常淋巴细胞,探讨其对原始细胞及异常淋巴细胞检测的价值。方法采用XN-20 WPC对出现原始细胞、异常淋巴细胞仪器报警的标本进行检测,将检测结果与人工镜检结果进行比较,分析XN-20 WPC与人工镜检结果的符合率,并对重复性与携带污染率进行评价。结果 XN-20 WPC与人工镜检对出现原始细胞/异常淋巴细胞、原始细胞及异常淋巴细胞仪器报警的检测符合率分别为83.3%、42.2%和42.9%。对未出现原始细胞、异常淋巴细胞仪器报警的标本,XN-20 WPC与人工镜检的符合率为97.4%。3份不同水平(低水平、中水平、高水平)原始细胞、异常淋巴细胞仪器报警标本的批内精密度[变异系数(CV)]分别为0.36%、1.47%、0.92%及1.02%、1.49%、0.73%,携带污染率均为0.02%。结论 XN-20 WPC的重复性良好,携带污染率较低,对外周血原始细胞及异常淋巴细胞具有一定的检测、识别能力,但还需结合人工镜检结果加以确认。Objective To investigate the role of XN-20 hematology analyzer white cell precursor channel（WPC） for the determinations of blast cells and abnormal lymphocytes in peripheral blood. Methods By XN-20 WPC,the determinations of blast cells and abnormal lymphocytes in peripheral blood were performed for specimens with Blasts and Abn Lympho alarms. The results were compared with those by manual microscopy. The consistency between XN-20 WPC and manual microscopy was evaluated. The repeatabilities and carry-over rates were evaluated as well. Results Compared with manual microscopy,the consistency rates of XN-20 WPC for specimens with Blasts/Abn Lympho,Blasts and Abn Lympho alarms were 83.3%,42.2% and 42.9%,respectively. The within-run precisions [coefficients of variation（CV）] of 3 specimens with Blasts and Abn Lympho alarms for different levels（low,middle and high levels） were 0.36%,1.47%,0.92% and 1.02%,1.49%,0.73%,respectively. The carryover rates were 0.02%. Conclusions The repeatability of XN-20 WPC is good,and the carry-over rates are low. XN-20 WPC can be used for the analysis of blast cells and abnormal lymphocytes in peripheral blood,which should be confirmed by manual microscopy further.

关 键 词：XN-20血液分析仪 白细胞前体通道 原始细胞 异常淋巴细胞 

分 类 号：R446.1[医药卫生—诊断学]