乳腺癌C-erbB-2基因启动子区CpG岛的甲基化状态与雄激素受体表达的相关性分析  

Correlation between the methylation status of C-erbB-2 gene promoter region CpG island and androgen receptor expression in breast cancer

作　　者：闫佩毅[1] 袁亚[2] 张骥[1] 朱琪[3] 邹玉涵[1] 王高莹 金姝[1] YAN Peiyi;YUAN Ya;ZHANG Ji;ZHU Qi;ZOU Yuhan;WANG Gaoying;JIN Shu(Department of Clinical Laboratory,Shanghai People＇s Hospital of Putuo District,Shanghai 200060,China;Department of Pathology,Shanghai People＇s Hospital of Putuo District,Shanghai 200060,China;Department of Surgery,Shanghai People＇s Hospital of Putuo District,Shanghai 200060,China)

机构地区：[1]上海市普陀区人民医院检验科,上海200060 [2]上海市普陀区人民医院病理科,上海200060 [3]上海市普陀区人民医院外科,上海200060

出　　处：《检验医学》2018年第6期549-555,共7页Laboratory Medicine

基　　金：江苏大学医学临床科技发展基金项目(JLY20140099)

摘　　要：目的探讨乳腺癌中雄激素受体(AR)与人表皮生长因子受体2(C-erbB-2)基因启动子区CpG岛甲基化状态的关系,并明确AR与乳腺癌预后的关系。方法分别采用甲基化特异性聚合酶链反应(MSP)和免疫组织化学(IHC)方法分析76例乳腺癌患者癌组织和55例乳腺良性肿瘤患者肿瘤组织C-erbB-2基因启动子区CpG岛的甲基化状态和AR的表达水平,结合临床病理资料分析AR不同表达状态与C-erbB-2基因启动子区CpG岛甲基化水平的关系,并进行预后分析。结果 AR阳性的乳腺癌组织C-erbB-2基因启动子区CpG岛甲基化的比例[74.58%(44/59)]显著高于AR阴性的乳腺癌组织[47.06%(8/17),P=0.032];C-erbB-2基因启动子区CpG岛的甲基化与AR蛋白的表达呈正相关(r=0.247,P=0.032),且乳腺癌AR阳性患者无瘤生存率显著高于AR阴性患者(P<0.05)。结论乳腺癌组织AR蛋白的表达状态可作为乳腺癌预后转归的预测依据之一;C-erbB-2基因启动子区CpG岛的甲基化与AR蛋白的表达状态呈正相关,为乳腺癌的激素治疗及预后转归的预测提供了新的理论依据。Objective To investigate the correlation between androgen receptor（AR） and the methylation status of human epidermal growth factor receptor（C-erbB-2） gene promoter region CpG island in breast cancer,and to study the relationship between AR and clinical prognosis. Methods A total of 76 patients with breast cancer and 55 patients with benign breast tumor were enrolled,and the methylation status of C-erbB-2 gene promoter region CpG island and the expression level of AR were determined by methylation-specific polymerase chain reaction（MSP）and immunohistochemistry（IHC）,respectively. Based on clinical and pathological data,the relationship between AR and the methylation status of C-erbB-2 gene promoter region CpG island was evaluated,and the prognosis analysis was performed. Results The methylation proportion of C-erbB-2 gene promoter region CpG island in AR positive breast cancer tissue [74.58%（44/59）] was higher than that in AR negative breast cancer tissue [47.06%（8/17）]（P=0.032）. The methylation of C-erbB-2 gene promoter region CpG island was positively correlated with AR expression（r=0.247,P=0.032）,and the tumor free survival rate of AR positive patients was higher than that of AR negative patients（P〈0.05）. Conclusions The expression status of AR in breast cancer tissue can be used as one of prognosis predictors. The methylation of C-erbB-2 gene promoter region CpG island is positively related to the expression status of AR,which provides a new reference for hormone therapy and breast cancer prognosis.

关 键 词：雄激素受体 人表皮生长因子受体2基因 甲基化 乳腺癌 

分 类 号：R446.62[医药卫生—诊断学]