Study on alantolactone-induced differentiation of mesenchymal stem cells intovascular cells  

作　　者：Yan-Jiao Lu Qiong Lu Ruo-Ke Su GangWang Rui Tan 

机构地区：[1]School of Life Sciences and Engineering, Southwest Jiaotong University, Chengdu, China [2]National EngineeringResearch Center for Biomaterials, Sichuan University, Chengdu, China [3]College of Medicine, Southwest JiaotongUniversity, Chengdu, China

出　　处：《Traditional Medicine Research》2018年第4期181-190,共10页TMR传统医学研究

摘　　要：To promote efficient screening of active angiogenic drugs from traditional medicines, we constructed a humanembryonic kidney-293 cell model using vascular endothelial growth factor （VEGF） gene promoter as the drug target. Inthis model, VEGF gene promoter may regulate the expression of the luciferase reporter gene by responding to thestimulation of drug molecules. This cell model allows rapid and efficient screening of vascular-inducing activecomponents from several drug monomer molecules. Furthermore, we used rat bone marrow mesenchymal stem cells（rMSCs） to conduct a preliminary study on the activity of alantolactone. Using simvastatin as a positive control, weinvestigated the effects of alantolactone on the expression of vascular-related cell marker molecules such as VEGF andα-smooth muscle actin （α-SMA） in rMSCs. According to our results, 0.1, 1, 3 and 5 μM of alantolactone upregulated thetranscriptional luciferase gene activity of VEGF promoter, and a significant difference from that in the control group wasobserved. Among them, 3μM of alantolactone showed the better effect than that of 3 μM of simvastatin （P = 0.036） andother concentrations of alantolactone and simvastatin showed similar effects. Compared with that in the control group,rMSCs induced with 1μM alantolactone for 3 days showed a significant increase in the relative mRNA expressions ofVEGF and α-SMA genes. However, these effect of 5 μM alantolactone were weaker than those of 5 μM simvastatin （P 〈0.05）; rMSCs treated with 1 μM alantolactone for 3 days showed brighter green fluorescence （FITC marker） of α-SMAand VEGF in situ expression than that observed in the control group and similar fluorescence intensity than that ofsimvastatin group in an immunoradiometric assay. The above results demonstrate the reliability of the highly efficientsystem for screening of active drug molecules and confirmed the vascular induction function of alantolactone at the geneand protein levels.

关 键 词：Alanlactone ANGIOGENESIS Stably transformed cell line rMSCs VEGF Α-SMA 

分 类 号：R917[医药卫生—药物分析学] R2-03[医药卫生—药学]