一种稻瘟病菌培养产孢培养基的配制与效比分析  

Preparation and Effect Ratio Analysis of a Medium for Pyricularia oryzae Culture and Sporulation

作　　者：吴斯骏[1] 孙强[2] 陈洪凡 杨增青 徐沛东[1] 杨迎青[1] 兰波[1] WU Si-jun 1, SUN Qiang 2, CHEN Hong-fan 1 et al(1. Plant Protection Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi 330200;2. Huangdao Entry-Exit Inspection and Quarantine Bureau, Qingdao, Shandong 26655)

机构地区：[1]江西省农业科学院植物保护研究所,江西南昌330200 [2]中华人民共和国黄岛出入境检验检疫局食品处,山东青岛266555

出　　处：《安徽农业科学》2018年第18期118-120,共3页Journal of Anhui Agricultural Sciences

基　　金：国家重点研发计划项目(2016YFD0200808);国家重点研发计划项目(2016YFD0300700);国家重点研发计划项目(2017YFD0301604);江西省水稻产业体系科研专项(JXARS-02-04)

摘　　要：[目的]筛选产孢效果好、成本低、操作简便的培养基。[方法]采用稻秸浸出液与PDA培养基为主要成分配制6组不同培养基,比较其稻瘟病菌的培养产孢情况。[结果]配组稻秸浸出液∶PDA培养基=1∶1具有最快的培养速率和最大的产孢量,并且与燕麦片培养基和大麦粒培养基相比在产孢效率和经济成本上也更具有优势。[结论]该新型培养基在水稻稻瘟病菌致病性与水稻品种抗瘟性鉴定研究中具有较高的应用价值。[Objective] The aim was to screen out a medium with good spores effect, low cost and simple operation. [Method] The culture effect of Pyricularia oryzae sporulation of six groups of culture medium which used rice straw leaching liquid and PDA culture medium as main components was compared. [Result] The formula rice straw extract ∶PDA medium = 1 ∶1 had the fastest culture rate and the largest amount of sporulation. Compared with oatmeal culture medium and barley culture medium, the culture medium also had advantages in the efficiency and economic cost of sporulation. [Conclusion] The new culture medium has good application value in the identification of pathogenicity and blast resistance of rice varieties.

关 键 词：稻瘟病菌 产孢 培养基 配制 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]