一测多评法测定血塞通胶囊中5种皂苷类成分的含量  

Simultaneous Determination of Five Saponins in Xuesaitong Capsules by QAMS

作　　者：姜志远[1] 刘世萍[2] 马妍妍[2] 李业雨 JIANG Zhiyuan;LIU Shiping;MA Yanyan;LI Yeyu(Disease Control and Prevention Center,Heilongjiang Farms ＆ Land Reclamation Administration,Harbin 150090,China;The First Affiliated Hospital of Harbin Medical University,Harbin 150001,China)

机构地区：[1]黑龙江省农垦总局疾病预防控制中心,黑龙江哈尔滨150090 [2]哈尔滨医科大学附属第一医院,黑龙江哈尔滨150001

出　　处：《中医药信息》2018年第4期42-45,共4页Information on Traditional Chinese Medicine

基　　金：黑龙江省教育厅资助项目(No.12541569)

摘　　要：目的:建立"一测多评法"(quantitative analysis single-marker,QAMS)同时测定血塞通胶囊中5种皂苷类成分的含量。方法:以人参皂苷Rg1为参照物,计算血塞通胶囊中的人参皂苷Rg1与三七皂苷R1、人参皂苷Re、Rb1及Rd之间的相对校正因子,分别采用外标法和"一测多评"法测定5种皂苷类成分的含量,并验证"一测多评"法的合理性、可行性和可重复性。结果:检测结果"一测多评"法与外标法无显著性差异,实验所得的相对校正因子可信。结论:本实验建立的"一测多评"法可行准确,可以更合理、快速地实现血塞通胶囊多种皂苷类成分的质量控制。Objective： To establish a method of quantitative analysis single-marker（ QAMS） for the contents determination of five saponins in Xuesaitong Capsules. Methods： Ginsenoside Rg1 was used as the internal standard,the relative correction factor（ RCF） of Notoginsenoside R1,Gginsenoside Re,Ginsenoside Rb1 and Ginsenoside Rd were calculated and evaluated. The external standard method and QAMS were used to determine the contents of 5 saponins. Also,the rationality,feasibility and repeatability of the QAMS method were verified by comparing the results obtained from two different methods. Results： There was no significant difference between the external standard method and QAMS method in terms of the testing results. Conclusion： It was concluded that the QAMS method can be accurately,rapidly and reasonably used as a new quality assessment model for ginsenosides in Xuesaitong Capsules.

关 键 词：血塞通胶囊 一测多评法 人参皂苷RG1 相对校正因子 

分 类 号：R285.5[医药卫生—中药学]