16/18型HPV和IMP3在宫颈脱落细胞中的表达和意义  

Expression of HPV-16/18 and Protein IMP3 in Cervical Exfoliated Cells

作　　者：吴倩[1] 易韦[1] 谢青梅[1] 龙昭玲[1] 寿娟 彭学勤[1] Wu Qian;Yi Wei;Xie Qingmei(Department of Pathology,Guizhou Provinical People＇s Hospital,Guiyang 550002,China)

机构地区：[1]贵州省人民医院(贵州医科大学附属人民医院)病理科,贵阳550002

出　　处：《华中科技大学学报（医学版）》2018年第3期321-324,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：贵州省科学技术厅联合基金资助项目(黔科合LH字[2015]7109号)

摘　　要：目的观察16/18型人乳头瘤病毒(HPV)和IMP3蛋白在宫颈液基薄层脱落细胞(LCT)中的表达,探讨其在宫颈癌筛查中的价值和意义。方法用原位杂交法检测163例宫颈细胞块中16/18型HPV DNA的表达情况,采用免疫组化法检测IMP3表达情况,并探讨其与宫颈病变程度的相关性。结果在不同细胞学类型病变中16/18型HPV阳性率不同,分别为NILM 10.00%、ASCUS 29.27%、LSIL 52.63%、HSIL 91.43%、SCC 100.00%;IMP3阳性率分别为NILM 2.50%、ASCUS 21.73%、LSIL 44.74%、HSIL 80.00%、SCC 88.89%。NILM、LSIL与HSIL组两两比较,二者的表达在组间均有显著差异(均P<0.05);且16/18型HPV及IMP3的表达均与宫颈病变程度呈正相关,随病变的级别增高而增高,在SCC中阳性率最高。结论 LCT行细胞块制作、联合检测16/18型HPV和IMP3的表达,可提高宫颈癌筛查的准确性,有效分流不同级别的患者,为指导临床治疗及评估预后奠定基础。Objective To observe the expression of human papilloma viruse 16/18（HPV16/HPV18）and IMP3 protein in cervical exfoliated cells from 163 cases,and to investigate the pathological significance in diagnosis of cervix diseases.MethodsThe expression of HPV-16/18 in cervical cells was detected by in situ hybridization;immunohistochemistry method was used to detect the expression of IMP3 in cervical exfoliated cells.Results The positive rate of HPV16/18 in NILM,ASCUS,LISL,HISL and SCC was 10.00%,29.27%,52.63%,91.43% and 100.00%,respectively.The positive expression rate of IMP3 in cervical exfoliated cells in NILM,ASCUS,LISL,HISL and SCC was 2.50%,21.73%,44.74%,80.00% and 88.89%.There were significant differences in the expression levels of HPV16/18 and IMP3 protein between NILM,LISL and HISL groups（P〈0.05）.The expression levels of HPV-16/18 and IMP3 were positively correlated with pathological grade of cervix lesion.The positive rate increased along with the increases in the pathological grade of cervix lesion,and the positive rate in SCC group was the highest.Conclusion The making of cell block from LCT,joint detection of HPV-16/18 and IMP3 protein can improve the detection rate,effectively identify the patient of different grades,and lay a foundation of guiding treatment and assessing prognosis.

关 键 词：人乳头瘤病毒 IMP3 细胞块 宫颈病变 原位杂交 免疫组化 

分 类 号：R711.74[医药卫生—妇产科学]