八宝景天乙酸乙酯提取物的抗菌活性和化学成分的研究  

Antibacterial Activity and Chemical Composition of Hylotelephium erythrostictum Ethyl Acetate Extracts

作　　者：尹秀梅[1] 王思宏[2,3] 李东浩[3] YIN Xiu-mei;WANG Si-hong;LI Dong-hao(College of Pharmacy,Yanbian University,Analysis and Inspection Center,Yanbian University,Key Laboratory of Natural Resource of the Changbai Mountain and Functional Molecules,Ministry of education,Yanbian Universit)

机构地区：[1]延边大学药学院 [2]延边大学测试中心 [3]延边大学长白山生物资源与功能分子教育部重点实验室,延吉133002

出　　处：《天然产物研究与开发》2018年第6期930-935,共6页Natural Product Research and Development

基　　金：国家自然科学基金(21365023)

摘　　要：以八宝景天(Hylotelephium erythrostictum)作为研究对象,得到石油醚、氯仿、乙酸乙酯、正丁醇、水层五种提取物,并用20种菌对提取物进行抗菌活性和最小抑菌浓度(MIC)实验,结果是乙酸乙酯层表现出较好的抗菌活性。进一步从乙酸乙酯层分离得到了11个化合物,分别为:芹菜素(1)、原儿茶酸(2)、没食子酸乙酯(3)、对羟基苯甲酸(4)、没食子酸(5)、香叶木素(6)、山奈酚(7)、4-乙酰-2-异丙基-5-甲基苯酚(8)、breynioside A(9)、1-苯-3,4-二氢-1H-异喹啉(10)、(1-苯-3,4-二氢-1H-异喹啉-2-基)丙烯酮(11)。其中化合物2,5~7首次从八宝景天中分离,化合物1,3,4,8~11首次从八宝属中分离。Hylotelephium erythrostictum was explored. Petroleumether,chloroform,ethylacetate,n-butanol and water extract are obtained,their antibacterial activity were investigated. The minimal inhibitory concentration（ MIC） of 5 solvent extracts for 20 tested strains are measured by microdilution method. The ethyl acetate extract exhibit the good antibacterial activity. Furthermore,11 compounds were isolated from the ethyl acetate extracts,including apigenin（ 1）,protocatechuic acid（ 2）,ethyl gallate（ 3）,4-hydroxybenzoic acid（ 4）,gallic acid（ 5）,diosmetin（ 6）,kaempferol（ 7）,4-acetyl-2-isopropyl-5-methylphenol（ 8）,breynioside A（ 9）,1,2,3,4-tetrahydro-1-phenyl-isoquinoline（ 10）,1-（ 3,4-dihydro-1-phenyl-2（ 1 H）-isoquinolinyl）-2-propen-1-one（ 11）. Compounds 2,5-7 are isolated from Hylotelephium erythrostictum for the first time. Compounds 1,3,4,8-11 are isolated from genusfor the first time.

关 键 词：八宝景天 抗菌活性 溶剂提取物 化学成分分离 

分 类 号：R932[医药卫生—生药学]