异质性万古霉素中介的金黄色葡萄球菌的分子特征  

Molecular Characteristics of Staphylococcus Aureus Mediated by Heterogeneous Vancomycin

作　　者：唐光敏[1] 周冠宇[1] 刘真真[1] Tang Guangmin;Zhou Guanyu;Liu Zhenzhen(Center for Infectious Diseases,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,China)

机构地区：[1]四川大学华西医院感染性疾病中心,四川成都610041

出　　处：《四川医学》2018年第5期480-484,共5页Sichuan Medical Journal

基　　金：四川省科技厅科技支撑项目(编号:2014KJT026-2014SZ)

摘　　要：目的了解我院异质性万古霉素中介的耐甲氧西林金黄色葡萄球菌的分布及分子学特征。方法对2015年10月至2016年5月我院临床分离的耐甲氧西林金黄色葡萄球菌,采用菌群分析策略-曲线下面积法(PAP-AUC)筛选异质性万古霉素中介的耐甲氧西林金黄色葡萄球菌;采用多重PCR法对hVISA进行agr、spa、SCC基因分型测定,对PCR产物进行测序确定spa分型。结果分离的79株MRSA经筛选获得9株h VISA;9株hVISA中有7株为agr-Ⅰ型,2株为agr-Ⅲ型;有6种spa克隆型,包括t437(4株),t114(1株)、t2310(1株)、t14062(1株)、t030(1株)、t16472(1株)。SCCmec分型以Ⅳa型为主,占55.56%,其次为Ⅲ型33.33%、Ⅱ型11.11%。结论我院hVISA检出率为11.39%,呈多克隆流行。Objective To investigate the distribution and molecular characteristics of methicillin-resistant Staphylococcus aureus mediated by heterogeneous vancomycin in our hospital.Methods Methicillin-resistant Staphylococcus aureus isolated in our hospital from October 2015 to May 2016 was screened for methicillin-resistant Staphylococcus aureus mediated by heterogeneous vancomycin by microflora analysis strategy-area under curve method（ PAP-AUC）; Multiplex PCR method was used to determine the genotyping of agr,spa and SCC in h VISA,and the PCR products were sequenced to determine the spa typing.Results After screening,9 strains of hVISA were isolated from 79 MRSA strains; Of the 9 strains of h VISA,7 were agr-I type and 2 were agr-Ⅲ type;There were 6 spa clone types,including t437（ 4 strains）,t114（ 1 strain） 、t2310（ 1 strain） 、t14062（ 1 strain） 、t030（ 1 strain） 、t16472（ 1 strain）.The SCCmec typing was mainly type Ⅳa,accounting for 55. 56%,followed by type Ⅲ 33. 33% and type Ⅱ 11. 11%.Conclusion The detection rate of hVISA in our hospital was 11. 39%,showing polyclonal prevalence.

关 键 词：异质性万古霉素中介 耐甲氧西林金黄色葡萄球菌 agr基因 spa基因 SCCme分型 

分 类 号：R378.11[医药卫生—病原生物学]