玉米幼苗盐胁迫条件下的生理响应机制  

Physiological Response Mechanism of Corn Seedlings under Salt Stress

作　　者：陈勋基[1] 陈果[1] 常晓春[1] 足木热木.吐尔逊 李建平[1] 郝晓燕[1] 高升旗[1] 黄全生[1] CHEN Xun-ji;CHEN Guo;CHANG Xiao-chun;Zumuremu Tursun;LI Jian-ping;HAO Xiao-yan;GAO Sheng-qi;HUANG Quan-sheng(Research Institute of Nuclear and Biotechnologies,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China)

机构地区：[1]新疆农业科学院核技术生物技术研究所,乌鲁木齐830091

出　　处：《新疆农业科学》2018年第4期627-634,共8页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区公益性科研院所基本科研业务经费项目"玉米耐盐性的全基因组关联性分析"(KY2015028);国家自然科学基金项目"玉米ZmCIPK42蛋白激酶参与盐胁迫反应的调控机理研究"~~

摘　　要：【目的】研究盐胁迫条件下玉米自交系和杂交种的响应,分析自交系和杂交种耐盐差异。在耐盐玉米新品种选育过程中,综合分析玉米自交系幼苗期对盐胁迫响应速度和植株体内Na^+、K^+离子含量,利用耐盐自交系为亲本,为筛选出耐盐的杂交组合。【方法】以玉米自交系B73、GEMS46以及B73×GEMS46杂交种为材料,进行耐盐性鉴定,分析比较自交系和杂交种在耐盐条件下生理指标变化趋势和最终差异,研究自交系对盐胁迫下杂种优势的贡献。【结果】玉米幼苗在3叶1心时用150 mmol/L NaCl处理植株是一个比较合理浓度;利用盐胁迫后植株的鲜重和干重以及植株Na^+、K^+离子含量是衡量植株耐盐性的较好的指标。在盐处理后,耐盐自交系的相对电导率、H_2O_2含量与杂交种变化趋势相同,耐盐自交系和杂交种前期对盐胁迫的响应较快,后期生理指标变化平缓,植株体内积累Na^+离子较小,表现为耐盐性。【结论】在耐盐玉米新品种选育过程中,综合分析玉米自交系幼苗期对盐胁迫响应速度和植株体内Na^+、K^+离子含量,利用耐盐自交系为亲本,可以较快地筛选出耐盐的杂交组合。【Objective】The identification of salt tolerance in maize inbred lines and hybrids is of great significance for breeding new varieties of salt-tolerant maize. In order to effectively analyze the response of maize inbred lines and hybrids under salt stress and to analyze the heterosis contribution of inbred lines,it is necessary to study the salt tolerance and heterosis performance of maize in-depth.【Method】The maize inbred lines B73,GEMS46 and B73 × GEMS46 F1 were used as testing materials to evaluate their salt tolerance.The trend and final difference of physiological indexes of inbred lines and hybrids under salt-tolerant conditions were analyzed and compared,thus reducing the contribution of inbred lines to heterosis under salt stress.【Result】The results showed that the treatment of 150 mmol/L NaCl in 3-leaf and 1-heart corn plants was a reasonable concentration. The fresh weight and dry weight of plant and Na＋and K＋content was criteria indicator. After salt treatment,the relative conductivity and H2 O2 content of salt-tolerant inbred lines had the same trend as that of hybrids,and the response of salt-tolerant inbred lines and hybrids to salt stress was rapid. The accumulation of Na＋in salt tolerance inbred and hybrid was stronger than those of the sensitive inbred lines. In the process of breeding new salt-tolerant maize varieties,the response speed of maize inbred lines to salt stress and the content of Na＋,K＋ions in the plants were analyzed synthetically. The salt-tolerant cross combinations could be quickly selected by using salt-tolerant inbred lines as parents. 【Conclusion】During the breeding of new salt-tolerant maize varieties the response speed to salt stress and the content of Na＋,K＋in seeding stage of maize inbred lines were comprehensivery analyzed by using salt-toleant inbred lines as parents which can be quickly screened Salt out of the hybrid combination.

关 键 词：玉米 自交系 耐盐 杂种优势 

分 类 号：S513[农业科学—作物学]