中药骨痹通消颗粒干预激素性股骨头坏死模型兔的细胞凋亡  

Gubi Tongxiao Granule effects on the cell apoptosis in a rabbit model of steroid-induced osteonecrosis of the femeral head

作　　者：周正新[1] 李文华[1] 朱磊[1] 韩士鼎 Zhou Zheng-xin;Li Wen-hua;Zhu Lei;Han Shi-ding(Department of Orthopedics,the First Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230031,Anhui Province,China)

机构地区：[1]安徽中医药大学第一附属医院骨伤科,安徽省合肥市230031

出　　处：《中国组织工程研究》2018年第20期3190-3194,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:股骨头坏死属于中医"骨痿"范畴,其疼痛是风寒瘀血阻滞造成的。而中药骨痹通消颗粒具有补肾壮骨、活血通络等功效,临床应用有效果,但其治疗机制尚不清晰。目的:分析中药骨痹通消颗粒对激素性股骨头坏死的治疗效果,并研究其对细胞凋亡的影响,为中药骨痹通消颗粒的临床应用提供一定的实验依据。方法:纳入54只健康家兔,随机分为对照组、模型组和治疗组,每组18只。对照组不作处理,模型组与治疗组均制备激素性股骨头坏死模型,造模成功后模型组生理盐水灌胃,治疗组给予骨痹通消颗粒灌胃治疗,1次/d,持续12周。治疗后4,8,12周,各组分别处死6只动物,取出双侧股骨头,检测股骨头坏死空骨陷窝率、细胞凋亡指数,并置于光镜下进行观察。结果与结论:(1)治疗后不同时间点对照组与治疗组股骨头坏死空骨陷窝率显著低于模型组(P<0.05);(2)光镜观察可见,对照组的股骨头坏死空骨陷窝情况基本维持不变;模型组第4周时存在明显的骨空陷窝,并随着时间的延长,数量逐渐增多,断裂现象明显;治疗组第4周时存在一定的骨空陷窝,随时间延长,空骨陷窝进一步减少;(3)治疗后不同时间点,对照组与治疗组的细胞凋亡指数显著低于模型组(P<0.05);(4)结果提示,中药骨痹通消颗粒治疗激素性股骨头坏死家兔模型效果显著,可有效减少细胞凋亡,并改善坏死部位的病理变化。BACKGROUND： Osteonecrosis of the femeral head（ONFH） is an atrophic debility of bones in Chinese medicine, the pain of which is caused by cold-induced blood stasis. Gubi Tongxiao Granule has the effects ofreinforcing the kidney to strength the bone and promoting blood circulation to remove obstruction in the channels. Its clinical efficacy is satisfactory, but the underlying mechanism is unclear. OBJECTIVE： To explore the curative efficacy of Gubi Tongxiao Granule for steroid-induced ONFH, and its effect on cell apoptosis, thus providing references for its clinical application. METHODS： Fifty-four healthy rabbits were randomly divided into model, treatment and control（no intervention） groups（n=18 per group）. The animal model of steroid-induced ONFH was established in the modeling and treatment groups, and then administered normal saline and Gubi Tongxiao Granule via gavage, respectively, once daily, for 12 consecutive weeks. At 4, 8 and 12 weeks after treatment, six animals in each group were sacrificed, and the bilateral femoral heads were removed. The empty bone lacuna rate and apoptotic index were determined, and the histological changes were observed under light microscope. RESULTS AND CONCLUSION： The empty bone lacuna rate in the control and treatment groups was significantly lower than that in the model group at different time points after treatment（P〈0.05）. There was no obvious change in necrotic empty bone lacuna in the control group. At 4 weeks after treatment, the empty bone lacunas were obvious in the model group, and increased in numbers and even broken with time increasing. At 4 weeks after treatment, empty bone lacunas were visible and increased in numbers in the treatment group. The apoptotic index in the control and treatment groups was significantly lower than that in the model group（P〈0.05）. These results indicate that Gubi Tongxiao Granule is effective for steroid-induced ONFH in rabbits, can effectively inhibit cell apoptosis and improve the pathological changes

关 键 词：中草药 模型 动物 股骨头坏死 细胞凋亡 组织工程 激素性股骨头坏死 中药骨痹通消颗粒 细胞凋亡指数 TUNEL法 空骨陷窝 甲基强的松龙 

分 类 号：R318[医药卫生—生物医学工程]