甲强龙联合脂多糖诱导成年犬股骨头动-静脉系统的组织病理学特点  

Histopathological characteristics of femoral head arteriovenous system induced by methylprednisolone and lipopolysaccharide in adult dogs

作　　者：张耀[1,2] 赵德伟[1,2] 王子华[2] 尉晓蔚[2] 田丰德[2] 杨帆[2] Zhang Yao;Zhao De-wei;Wang Zi-hua;Yu Xiao-wei;Tian Feng-de;Yang Fan(School of Biomedical Engineering,Dalian University of Technology,Dalian 116024,Liaoning Province,China;Department of Orthopedics,Affiliated Zhongshan Hospital of Dalian University,Dalian 116001,Liaoning Province,China)

机构地区：[1]大连理工大学生物医学工程学院,辽宁省大连市116024 [2]大连大学附属中山医院骨科,辽宁省大连市116001

出　　处：《中国组织工程研究》2018年第20期3195-3200,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81601885)~~

摘　　要：背景:糖皮质激素类的诸多作用机制对于与骨坏死的病理过程的启动有密切关系,然而对于激素性骨坏死时股骨头动、静脉血管在不同时段发生的病理形态学变化的区别仍缺少相关记述。目的:观察大剂量皮质激素联合脂多糖诱导下的成年犬在不同时段其动脉和静脉系统的组织病理形态学变化,以讨论这种变化对骨坏死发生和发展造成的可能影响。方法:将健康成年犬15只随机分为4,8周观察组及对照组,每组5只(10髋)。4,8周观察组行静脉注射脂多糖10 g/kg,于24 h后肌注甲强龙20 mg/kg,连续注射3次,每次间隔24 h,干预后4,8周麻醉处死,取股骨头行双侧病理切片染色,对骨内及支持带动静脉血管缩窄度进行分析测量。结果与结论:诱导4,8周后,犬骨内静脉的结构形态发生明显变化,表现为正常型静脉结构显著减少,而中度狭窄型、阻塞塌陷型静脉结构占比显著升高。在骨内动脉系统,8周观察组相对其他2组骨内区域动脉血管内膜相对增厚,血管缩窄程度高(P<0.05)。结果证实,在皮质激素联合脂多糖诱导下,成年犬股骨头动脉和静脉系统出现病理变化的时间节点存在差异。静脉系统在药物诱导早期(4周)最先呈现出病理变化,而动脉系统病理变化出现在药物诱导8周后。BACKGROUND： Many mechanisms of action of glucocorticoids are closely related to the occurrence of osteonecrosis. However, the differences in pathomorphological changes between femoral head arteries and veins at different periods of steroid-induced osteonecrosis are rarely reported.OBJECTIVE： To observe the histopathological changes of the arterial and venous system in adult dogs induced by high-dose corticosteroid combined with lipopolysaccharide, and to explore effects of these changes on the occurrence and development of osteonecrosis. METHODS： Fifteen healthy adult dogs were randomly divided into, 4-and 8-week observation groups and control group（n=5（10 hips） per group）. The dogs in the observation groups underwent venous injection of 10 g/kg lipopolysaccharide, then intramuscular injection of 20 mg/kg methylprednisolone after 24 hours, for 3 times, with 24 hours in between. Afterwards, the dogs were sacrificed at 4 and 8 weeks respectively. The bilateral femoral heads were removed for pathological observation. The stenotic degree of arteries and veins in the bone and supporting band was measured. RESULTS AND CONCLUSION： At 4 and 8 weeks after induction, the structure and morphology of normal intraosseous vein decreased significantly, while the proportion of moderate-stenotic and blocked-collapsed veins increased significantly. Compared with the 4-week observation and control groups, in the 8-week observation group, the thickness of arterial intima in the bone was significantly increased, and the stenotic degree was significantly increased（P〈0.05）. These results indicate that there is difference in the occurrence of pathological changes between femoral artery and venous system in adult dogs under the induction of corticosteroids combined with lipopolysaccharide. The venous system presents the pathological changes at the early stage after induction（4 weeks）, and the pathological changes occurred in the arterial system at 8 weeks.

关 键 词：组织工程 股骨头坏死 脂多糖 动物实验 骨内动静脉 糖皮质激素-脂多糖诱导 组织学 病理学 血管缩窄度 

分 类 号：R318[医药卫生—生物医学工程]