四氯化碳加乙醇复合法诱导肝硬化大鼠模型的建立及验证  

A rat model of liver cirrhosis induced by the combination of carbon terachloride and ethanol: establishment and verification

作　　者：宾文婷[1] 常加松[1] 吴剑平[2] Bin Wen-ting;Chang Jia-song;Wu Jian-ping(Department of Human Anatomy and Histoembryology;Laboratory Animal Center,Nanjing University of Chinese Medicine,Nanjing 210023,Jiangsu Province,China)

机构地区：[1]南京中医药大学人体解剖与组织胚胎学系,江苏省南京市210023 [2]南京中医药大学实验动物中心,江苏省南京市210023

出　　处：《中国组织工程研究》2018年第20期3224-3229,共6页Chinese Journal of Tissue Engineering Research

基　　金：江苏省高校自然科学研究计划资助项目(13KJD310001)~~

摘　　要：背景:建立稳定、可靠、易操作又高效的肝硬化动物模型是进行肝硬化防治研究的前提条件。目的:探寻一种理想的CCl_4诱导大鼠肝硬化模型的造模方法。方法:将Wistar大鼠随机分为正常对照组(n=8)、模型A组(n=21)及模型B组(n=21),正常对照组左右腹部皮下交替注射橄榄油,每4d一次;模型A组用传统型方法,左右腹部皮下交替注射3 m L/kg 50%CCl_4橄榄油溶液,每4 d一次,以10%乙醇溶液(以二锅头白酒、蒸馏水配制)为其惟一饮用水;模型B组用改良的CCl_4加乙醇复合法诱导大鼠肝硬化模型,左右腹部皮下交替注射50%CCl_4橄榄油溶液,最初4次及最末3次剂量为5 m L/kg,其余为3 m L/kg,每4 d一次,以乙醇溶液(以二锅头白酒、蒸馏水配制)为其惟一饮用水,乙醇浓度从5%逐渐过渡为10%。造模时间9周。结果与结论:与正常对照组相比,模型A、B组大鼠体质量增加缓慢,与模型A组相比,模型B组体质量波动较小。模型A、B两组丙氨酸氨基转移酶和天门冬氨酸氨基转移酶均明显高于正常对照组,模型B组丙氨酸氨基转移酶水平高于模型A组,而模型A、B两组天门冬氨酸氨基转移酶水平差异无显著性意义。模型B组与正常对照组存活率差异无显著性意义,且模型B组比模型A组假小叶形成率高。提示用改良的CCl_4加乙醇复合法造模的大鼠肝硬化模型死亡率低且成模率高。BACKGROUND： Establishing a stable, reliable, easily handling and highly efficient animal model of liver cirrhosis is the prerequisite for the study on the prevention and treatment of the disease.OBJECTIVE： To establish and evaluate an ideal means to induce liver cirrhosis in rats by carbon tetrachloride（CCl_4）. METHODS： Wistar rats were randomly allotted into control group（n=8）, group A（n=21） and group B（n=21）. Olive oil was injected subcutaneously into both sides of the abdomen of control rats every 4 days; in the group A, 50% CCl_4 in olive oil was injected subcutaneously into the abdomen with a dosage of 3 m L/kg every 4 days, and a 10% ethanol solution（Erguotou liquor and distilled water） constituted the only liquid drank; in the group B, 50% CCl_4 in olive oil was injected subcutaneously into the abdomen every four days, the first four and final three doses were 5 m L/kg, and the other doses were 3 m L/kg, and a gradually transition from 5% to 10% ethanol solution（Erguotou liquor and distilled water）, constituted the only liquid drank. The modeling time was 9 weeks. RESULTS AND CONCLUSION： There was a slower increase in the rat body mass in the groups A and B compared with the control group, especially in the group B. The serum levels of alanine aminotransferase and aspartate aminotransferase in the groups A and B were significantly higher than those in the control group. The level of alanine aminotransferase in the group B was significantly higher than that in the group A, but there was no significant difference in the aspartate aminotransferase level between the groups A and B. The cirrhosis with pseudolobules rate and success rate in the group B were higher than those in the group A. There was no significant difference in survival rate between group B and control group. That is to say, the improved method of inducing cirrhotic model in rats by CCl_4 combined with ethanol shows a high rate of success and a low rate of mortality.

关 键 词：肝 肝硬化 四氯化碳 组织工程 乙醇 模型 实验动物 大鼠 肝脏 

分 类 号：R318[医药卫生—生物医学工程]