高效液相色谱法同时测定抗骨增生丸中柚皮苷和淫羊藿苷的含量  

Simultaneous Determination of Naringin and Icariin in Anti-hyperostosis Pills by HPLC

作　　者：罗亚玲 李冰 吴强 LUO Yaling;LI Bing;WU Qiang(Sichuan Provincial Institute for Food and Drug Control,Chengdu 611731,China;Leshan Traditional Chinese Medicine Hospital,Leshan 614000,China)

机构地区：[1]四川省食品药品检验检测院,四川成都611731 [2]乐山市中医医院,四川乐山614000

出　　处：《成都大学学报（自然科学版）》2018年第2期147-149,158,共4页Journal of Chengdu University（Natural Science Edition）

摘　　要：建立了以高效液相色谱(HPLC)法同时测定抗骨质增生丸中柚皮苷及淫羊藿苷含量的方法.采用C18色谱柱(250×4.6 mm,5μm),以水为流动相A,乙腈为流动相B,梯度洗脱,流速为1.0 m L/min,检测波长分别为282 nm(柚皮苷)、270 nm(淫羊藿苷).结果显示,新建的含量测定方法专属性强,线性关系良好,柚皮苷与淫羊藿苷加样回收率分别为96.90%和99.71%,RSD分别为1.52%和1.80%.实验表明,本法简便、专属性强、重复性好,能有效控制抗骨增生丸的质量.The paper aims to establish an HPLC method for determining naringin and icariinin in antihyperostosis pills. The separation is performed on a C18 column（250 × 4. 6 mm,5 μm） by gradient elution with water as mobile phase A and acetonitrile as phase B. The detection wavelength is at 282 nm（ naringin） and 270 nm（ icariin）; the flow rate is 1. 0 m L/min. The results show that the new method of content determination is highly specific. There is a good linear relationship. The sample recoveries of the analytes are 96. 90% for naringin and 99. 71% for icariin. The RSD is 1. 52% for naringin and 1. 80% for icariin. It＇s concluded that this method is convenient with strong specificity and good repeatability. It can be used to control the quality of anti-hyperostosis pills.

关 键 词：抗骨增生丸 柚皮苷 淫羊藿苷 含量测定 高效液相色谱 

分 类 号：R286.0[医药卫生—中药学] O657.72[医药卫生—中医学]